Prognostic significance of ALCAM (CD166/MEMD) expression in cutaneous melanoma patients
Prognostic significance of ALCAM (CD166/MEMD) expression in cutaneous melanoma patients
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DOI：
10.1186/s13000-015-0331-z
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发表时间：
2015-07-02
影响因子：
2.6
通讯作者：
Matkowski, Rafal
中科院分区：
文献类型：
--
作者：
Donizy, Piotr;Zietek, Marcin;Matkowski, Rafal
背景：ALCAM （activated leukocyte cell adhesion molecule, CD166， MEMD）是免疫球蛋白超家族（immune globulin superfamily, Ig-SF）中的跨膜蛋白，在人类恶性黑色素瘤的进展和局部及远处转移的形成中起重要作用。利用黑色素瘤细胞系的研究表明，ALCAM过表达与细胞聚集增加和形成细胞巢的能力直接相关。该研究的目的是评估ALCAM在原发性皮肤黑色素瘤和区域淋巴结转移病变中的表达和细胞内定位。同时，通过5年观察，评估ALCAM在原发肿瘤细胞和转移灶细胞中的表达对预后的意义。方法：对104例原发性皮肤黑色素瘤和16例局部淋巴结转移瘤进行福尔马林固定石蜡包埋组织，免疫组化检测ALCAM的表达。结果：我们证明，与肿瘤间室ALCAM免疫反应性较低的患者相比，原发性黑色素瘤细胞中ALCAM高表达（IRS >= 8）与不良预后密切相关，就癌症特异性总生存期（CSOS） （P = 0.001）和无病生存期（DFS） （P < 0.001）而言。Clark评分显示，淋巴结转移灶ALCAM免疫反应性较低与原发肿瘤黑色素瘤浸润深度有统计学意义（P = 0.032）。同时发现ALCAM表达降低（IRS）
Background: ALCAM (activated leukocyte cell adhesion molecule, CD166, MEMD) is a transmembrane protein of immunoglobulin superfamily (Ig-SF) and plays an important role in human malignant melanoma progression and formation of locoregional and distant metastases. The study using melanoma cell lines showed that overexpression of ALCAM is directly related with the increase of cytoaggregation and the ability to form cell nests. The aim of the study was to assess the expression and intracellular localization of ALCAM in primary skin melanomas and metastatic lesions from regional lymph nodes. Also, prognostic significance of ALCAM expression in primary tumor cells and metastatic lesion cells was evaluated in the context of 5-year observation.Methods: Formalin-fixed paraffin-embedded tissue specimens from 104 primary cutaneous melanomas and 16 regional lymph nodes metastases were studied for the expression of ALCAM measured by immunohistochemistry.Results: We demonstrate that high ALCAM expression in primary melanoma cells (IRS >= 8) is strongly correlated with unfavorable prognosis as compared with patients with lower ALCAM immunoreactivity in tumor compartment as regards cancer specific overall survival (CSOS) (P = 0.001) and disease free survival (DFS) (P < 0.001). Additionally lower ALCAM immunoreactivity in nodal metastatic foci was significantly statistically correlated with deeper melanoma invasion in the primary tumor according to Clark scale (P = 0.032). It was also found that decreased ALCAM expression (IRS