Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017-2018 in Minas Gerais State, Brazil
Detection of Yellow Fever Virus in Sylvatic Mosquitoes during Disease Outbreaks of 2017-2018 in Minas Gerais State, Brazil
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DOI：
10.3390/insects10050136
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发表时间：
2019-05-01
期刊：
影响因子：
3
通讯作者：
Andrade Filho, Jose Dilermando
中科院分区：
文献类型：
--
作者：
Pinheiro, Guilherme Garcia;Rocha, Marcele Neves;Andrade Filho, Jose Dilermando
巴西近年来经历了几次虫媒病毒暴发，其中黄热病尤为突出。米纳斯吉拉斯州在2017年和2018年面临森林黄热病暴发，确诊病例1002例，死亡340例。这项工作介绍了在巴西贝洛奥里藏特大都市地区的两个保护区的蚊子中检测黄热病病毒的调查结果。2017年9月至2018年5月共采集蚊虫20种867只，其中数量最多的是Psorophora(Janthinosoma)ferox(von Humboldt，1819)(31.3%)、Limatus duhamii Theobald，1901(19.1%)和Haemagogus(Haemagogus)jansinomys Dyar，1921(18.2%)。从蚊子中提取总RNA，用于实时PCR分析黄热病、基孔肯雅热、玛雅罗病毒、寨卡病毒和登革热病毒。汞人群黄热病感染率为8.2%。简蒂诺米斯(13只蚊子)，这是巴西森林黄热病的主要媒介。除了调查这些保护单位的蚊子动物群外，这项工作还表明了监测大型城市中心附近病毒传播的重要性。
Brazil has experienced several arbovirus outbreaks in recent years, among which yellow fever stands out. The state of Minas Gerais faced outbreaks of sylvatic yellow fever in 2017 and 2018, with 1002 confirmed cases and 340 deaths. This work presents the results of survey efforts to detect the yellow fever virus in mosquitoes from two conservation areas in the metropolitan region of Belo Horizonte, Brazil. A total of 867 mosquitoes of 20 species were collected between September 2017 and May 2018, the most abundant being Psorophora (Janthinosoma) ferox (von Humboldt, 1819) (31.3%), Limatus durhamii Theobald, 1901 (19.1%) and Haemagogus (Haemagogus) janthinomys Dyar, 1921 (18.2%). Total RNA was extracted from the mosquitoes for real-time PCR analysis for yellow fever, chikungunya, mayaro, Zika and dengue viruses. The yellow fever infection rate was 8.2% for Hg. janthinomys (13 mosquitoes), which is the main vector of sylvatic yellow fever in Brazil. In addition to surveying the mosquito fauna of these conservation units, this work demonstrates the importance of monitoring the circulation of viruses near large urban centers.