FUNCTIONAL AND ANATOMICAL ORGANIZATION OF CARDIOVASCULAR PRESSOR AND DEPRESSOR SITES IN THE LATERAL HYPOTHALAMIC AREA .2. ASCENDING PROJECTIONS
FUNCTIONAL AND ANATOMICAL ORGANIZATION OF CARDIOVASCULAR PRESSOR AND DEPRESSOR SITES IN THE LATERAL HYPOTHALAMIC AREA .2. ASCENDING PROJECTIONS
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DOI：
10.1002/cne.903300310
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发表时间：
1993-04-15
影响因子：
2.5
通讯作者：
CECHETTO, DF
中科院分区：
文献类型：
--
作者：
ALLEN, GV;CECHETTO, DF
采用微量注射l -谷氨酸或D， l -同型半胱氨酸选择性刺激下丘脑外侧区（LHA）的细胞体。随后将菜豆-白细胞凝集素离子电泳注射或小麦胚芽凝集素-辣根过氧化物酶压力注射到含有确定的加压和抑制位点的区域，并追踪它们与前脑和大脑皮层的联系。结果表明，血压（10- 45mmhg）和心率（20- 70bpm）的降低可以从LHA的结节（LHAt）和后（LHAp）部位引起，这些区域向上投射到岛叶皮质、前脑腹侧（包括Broca复合体的隔对角带）、腹侧palladium、nominata物质、杏仁核和外侧视前区。相反，血压（10-40毫米汞柱）和心率（20-70 bpm）的升高主要由位于穹窿周围的皮质周围区（PFA）的神经元引起。将示踪剂注射到该区域产生的末端标记与将示踪剂注射到LHA的抑制位点所见的模式明显不同。此外，向PFA前部注射示踪剂所见的顺行标记模式与向PFA后部注射示踪剂所见的不同。在PFA前部注射示踪剂可在下丘脑视前内侧区和室旁旁细胞旁核形成密集的末端标记，而在PFA后部注射示踪剂可在中隔外侧核、伏隔核、终纹床核以及下丘脑视前内侧区和室旁细胞旁核形成密集的末端标记。结果表明，大鼠下丘脑后外侧包含两个具有特定心血管功能的区域，并与间脑、前脑和皮质结构有高度组织的联系。
Microinjections of L-glutamate or D,L-homocysteic acid were used to stimulate cell bodies in the region of the lateral hypothalamic area (LHA) selectively. Subsequent iontophoretic injections of Phaseolus vulgaris-leucoagglutinin or pressure injections of wheat germ agglutinin-horseradish peroxidase were made into regions containing identified pressor and depressor sites and their connections with the forebrain and cerebral cortex were traced.The results indicate that decreases in blood pressure (10-45 mm Hg) and heart rate (20-70 bpm) could be elicited from tuberal (LHAt) and posterior (LHAp) sites in the LHA and that these regions have ascending projections to the insular cortex, the ventral forebrain including the septal-diagonal band of Broca complex, the ventral palladium, substantia innominata, amygdala, and the lateral preoptic area. In contrast, increases in blood pressure (10-40 mm Hg) and heart rate (20-70 bpm) were elicited primarily from neurons located adjacent to the fornix in the perifornical area (PFA). Injections of tract tracers into this region produced terminal labeling that differed markedly from the pattern seen following injections of tracer into depressor sites in the LHA. In addition, the pattern of anterograde labeling seen following injections of tracer into the anterior PFA differed from that seen following injections of tracer into the posterior PFA. Injections of tracer into the anterior PFA resulted in dense terminal labeling in the medial preoptic area and the parvicellular paraventricular nucleus of the hypothalamus whereas injections into the posterior PFA resulted in dense terminal labeling in the lateral septal nucleus, nucleus accumbens, bed nucleus of the stria terminalis, as well as the medial preoptic area and the parvocellular paraventricular nucleus of the hypothalamus.The results demonstrate that the posterolateral hypothalamus of the rat contains two regions with specific cardiovascular function and highly organized connections with diencephalic, forebrain, and cortical structures.