Total arsenic concentrations in toenails quantified by two techniques provide a useful biomarker of chronic arsenic exposure in drinking water
Total arsenic concentrations in toenails quantified by two techniques provide a useful biomarker of chronic arsenic exposure in drinking water
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DOI：
10.1016/j.envres.2005.08.004
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发表时间：
2006-06-01
影响因子：
8.3
通讯作者：
Thomas, David J.
中科院分区：
文献类型：
--
作者：
Adair, Blakely M.;Hudgens, Edward E.;Thomas, David J.
对任何感兴趣的污染物进行准确定量对于支持风险评估的暴露评估和代谢研究至关重要。内华达州砷暴露评估研究的初步步骤是量化组织中的总砷 (TA) 浓度，作为暴露的生物标志物。这项研究的参与者 (n = 95) 年龄至少为 45 岁，在该地区居住了 20 多年，并且接触过各种砷浓度的饮用水 (3-2100ppb)。通过氢化物发生原子荧光光谱法 (HG-AFS) 测定的血液、尿液和脚趾甲中 TA 的浓度范围分别为低于检测值至 0.03、0.76 和 12ppm；血液中的TA很少超过检测限。为了进行比较，通过中子活化分析 (NAA) 测定的脚趾甲中的 TA 范围从低于检测值到 16ppm。脚趾甲和饮用水中的 TA 浓度之间存在显着（P < 0.0001）正回归（调整后的 r(2) = 0.3557 HG-AFS，调整后的 r(2) = 0.3922 NAA）；尿液中的 TAs 浓度不能用饮用水 As 来描述（调整后的 r(2) = 0.0170，P = 0.1369）。 HGAFS 和 NAA 对脚趾甲中 TA 的分析得出了高度一致的估计值（r = 0.7977，P < 0.0001）。这些结果表明，在本研究中，与尿液相比，脚趾甲是慢性砷暴露的更好的生物标志物，因为脚趾甲中砷的隔离提供了随时间的暴露的整合，而尿液中不会出现这种情况。 (c) 2005 Elsevier Inc. 保留所有权利。
Accurate quantitation of any contaminant of interest is critical for exposure assessment and metabolism studies that support risk assessment. A preliminary step in an arsenic exposure assessment study in Nevada quantified total arsenic (TAs) concentrations in tissues as biomarkers of exposure. Participants in this study (n = 95) were at least 45 years old, had lived in the area for more than 20 years, and were exposed to a wide range of arsenic concentrations in drinking water (3-2100ppb). Concentrations of TAs in blood, urine, and toenails determined by hydride generation-atomic fluorescence spectrometry (HG-AFS) ranged from below detection to 0.03, 0.76, and 12ppm, respectively; TAs in blood rarely exceeded the limit of detection. For comparison, TAs in toenails determined by neutron activation analysis (NAA) ranged from below detection to 16ppm. Significant (P < 0.0001) positive regressions were seen between the TAs concentration in toenails and in drinking water (adjusted r(2) = 0.3557 HG-AFS, adjusted r(2) = 0.3922 NAA); TAs concentrations in urine were not described by drinking water As (adjusted r(2) = 0.0170, P = 0.1369). Analyses of TAs in toenails by HGAFS and NAA yielded highly concordant estimates (r = 0.7977, P < 0.0001). These results suggest that toenails are a better biomarker of chronic As exposure than urine in the current study, because the sequestration of As in toenails provides an integration of exposure over time that does not occur in urine. (c) 2005 Elsevier Inc. All rights reserved.