The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum
The preovulatory rise of ovarian ornithine decarboxylase is required for progesterone secretion by the corpus luteum
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DOI：
10.1016/s0006-291x(02)00191-2
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发表时间：
2002-04-26
影响因子：
3.1
通讯作者：
Peñafiel, R
中科院分区：
文献类型：
--
作者：
Bastida, CM;Tejada, F;Peñafiel, R
动情周期的发情间期期间，卵巢黄体酮分泌是由颗粒细胞和卵泡膜细胞衍生的黄体细胞在排卵后的分化过程后产生的。我们的结果表明，阻断卵巢鸟氨酸脱羧酶（ODC）的排卵前升高。多胺生物合成中的关键酶。通过使用特定抑制剂 α-二氟甲基鸟氨酸 (DFMO) 进行治疗，会导致卵巢孕酮含量显着下降，并且在接下来的发情间期期间，该激素的血浆水平会急剧下降。尽管黄体生成素和卵泡刺激素与 DFMO 同时给予，但仍产生相同的抑制作用。另一方面。在动情周期的不同时期给小鼠施用人绒毛膜促性腺激素后观察到的血浆孕酮水平急剧上升并未受到DFMO施用的影响。我们的结果表明，尽管 ODC 水平升高并不是急性卵巢类固醇生成所必需的。在发情前期晚上观察到的卵巢 ODC 活性的排卵前峰值可能对于小鼠发情周期的发情间期期间黄体黄体的组成性类固醇生成途径和孕酮分泌的建立至关重要。 (C) 2002 年爱思唯尔科学（美国）。版权所有。
Ovarian progesterone secretion during the diestrus stage of the estrous cycle is produced by luteal cells derived from granulosa and thecal cells after the differentiation process that follows ovulation. Our results show that blockade of the preovulatory rise of ovarian ornithine decarboxylase (ODC). a key enzyme in polyamine biosynthesis. by treatment with the specific inhibitor alpha-difluoromethylornithine (DFMO) leads to a significant decrease in the ovarian progesterone content and a dramatic fall in the plasma levels of this hormone during the following diestrus. The same inhibition was produced in spite of the fact that both luteinizing and follicle stimulating hormones were given concomitantly with DFMO. On the other hand. the acute rise in the plasma progesterone levels observed after administration of human chorionic gonadotropin to mice at different periods of the estrous cycle was not affected by DFMO administration. Our results indicate that although elevated levels of ODC are not required for acute ovarian steroidogenesis. the preovulatory peak of ovarian ODC activity observed in the evening of proestrus may be critical for the establishment of a constitutive steroidogenic pathway and progesterone secretion by the corpus luteum during the diestrus stage of the murine estrous Cycle. (C) 2002 Elsevier Science (USA). All rights reserved.