MHC-SPECIFIC GRAFT-PROTECTIVE AND DELAYED-TYPE HYPERSENSITIVITY (DTH) SUPPRESSIVE ACTIVITY OF A CD4+ CD8+, ALPHA-BETA-T-CELL RECEPTOR (TCR) POSITIVE LYMPHOMA ISOLATED FROM A TOLERANT MOUSE
MHC-SPECIFIC GRAFT-PROTECTIVE AND DELAYED-TYPE HYPERSENSITIVITY (DTH) SUPPRESSIVE ACTIVITY OF A CD4+ CD8+, ALPHA-BETA-T-CELL RECEPTOR (TCR) POSITIVE LYMPHOMA ISOLATED FROM A TOLERANT MOUSE
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DOI：
10.1016/s0171-2985(11)80496-1
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发表时间：
1993-06-01
期刊：
影响因子：
2.8
通讯作者：
VEGH, P
中科院分区：
文献类型：
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作者：
JANOSSY, T;BARANYI, L;VEGH, P
通过新生儿注射（CBAxA）F1脾细胞诱导对CBA （H-2k）组织相容性抗原的永久移植耐受，在A （H-2a）小鼠中发现并分离了两个淋巴瘤。它们被证明是受体来源，可转移到同基因A小鼠，生长为弥散性淋巴瘤（L33和L46），并迅速杀死受体。细胞表面抗原分析显示，两种淋巴瘤均具有不成熟T细胞表型[Thy-1+， CD5+, CD3low, tcrα (low), CD4low, CD8high，热稳定抗原（HSA）阳性，CD44-， MHC II类-，CD45R7-, sIg-, Gr-1-, CD11b-]。腹腔注射具有活的L33淋巴瘤细胞的同基因A小鼠，导致CBA和mhc相同的B10的平均生存时间呈剂量依赖性显著延长。BR同种异体皮肤移植物与非淋巴瘤对照中适当移植物的存活率比较。第三方mhc不相容BIO （H-2b）和B10的生存时间。在接种L33细胞的A小鼠中，D2 （H-2d）同种异体移植物仅略微延长。体外丝裂霉素C （MMC）预处理可阻断L33细胞的增殖能力，但不影响移植物保护作用。此外，A小鼠接种L33淋巴瘤可显著抑制小鼠对致敏CBA组织相容性抗原的DTH反应。相比之下，L46淋巴瘤对CBA同种异体移植物的存活和DTH反应性没有影响。这些数据表明，来自新生耐受小鼠的CD4+ cd8tcralpha + L33 T细胞淋巴瘤对同基因小鼠体内对诱导耐受（MHC I类）异体抗原的反应性具有特异性免疫抑制作用。
Two lymphomas were found in, and isolated from A (H-2a) mice in which permanent transplantation tolerance was induced to CBA (H-2k) histocompatibility antigens by the neonatal injection of (CBAxA)F1 spleen cells. They proved to be of recipient origin and were transferable to syngeneic A mice, growing as disseminated lymphomas (L33 and L46) and killing the recipients rapidly. Analysis of the cell surface antigens disclosed that both lymphomas had an immature T cell phenotype [Thy-1+, CD5+, CD3low, TCRalphabeta(low), CD4low, CD8high, heat-stable antigen (HSA) positive, and CD44-, MHC class II-, CD45R7-, sIg-, Gr-1-, CD11b-].Intraperitoneal (i.p.) injection of syngeneic A mice with viable L33 lymphoma cells resulted in a dose-dependent, significant prolongation of the mean survival times of CBA and MHC-identical B10.BR skin allografts as compared to the survival of appropriate grafts in non-lymphoma-bearing controls. The survival times of third party MHC-incompatible BIO (H-2b) and B10.D2 (H-2d) allografts were only slightly prolonged in A mice inoculated with L33 cells. The graft-protective effect was not abrogated if the proliferative capacity of the L33 cells was blocked by in vitro mitomycin C (MMC) pretreatment. Furthermore, the inoculation of L33 lymphoma into A mice significantly inhibited their DTH response to the sensitizing CBA histocompatibility antigens. In contrast, the L46 lymphoma had no effect on the survival of CBA allografts and the DTH reactivity. These data suggest that the CD4+CD8TCRalphabeta+ L33 T cell lymphoma originating from a neonatally tolerant mouse has a specific immunosuppressive effect on the in vivo reactivity of syngeneic mice to the tolerance-inducing (MHC class I) alloantigens.