Cerebral concussion in rats rapidly induces hypothalamic-specific effects on opiate and cholinergic receptors
Cerebral concussion in rats rapidly induces hypothalamic-specific effects on opiate and cholinergic receptors
复制标题
大鼠脑震荡迅速诱导下丘脑对阿片和胆碱能受体的特异性影响
DOI：
10.1016/0006-8993(81)90835-0
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
D. Parkinson
中科院分区：
文献类型：
--
作者：
M. West;F. LaBella;V. Havlicek;D. Parkinson
有意识的雄性成年老鼠被一把装有弹簧的手枪射出的钝镖击中了枕骨，造成了脑震荡。动物在2秒内被斩首，大脑被迅速移除，在冷缓冲液中均匀化几个大脑区域，并进行放射性受体测定。[3H]纳洛酮与[3H]QNB在下丘脑的结合增加了20%，而在杏仁核、纹状体、海马、皮层、中脑和后脑的结合没有增加。对于脑震荡后5-10秒死亡的动物，当正常的意识行为恢复时，对照组和脑震荡大鼠之间的结合没有变化。[3H]螺哌啶醇与[3H]沃巴因的结合不受脑震荡的影响。为了排除非特异性应激引起的变化，一组大鼠的下半身受到了打击。与击打头部相比，击打身体后[3H]QNB与下丘脑组织的结合与对照组相比没有变化。研究结果表明，内源性内啡肽和乙酰胆碱与各自受体的结合减少是脑震荡的结果。内源性递质结合的短暂性缺陷可能暂时中断与意识状态有关的下丘脑回路。
Conscious male adult rats were concussed by a blow to the occiput with a blunt dart, shot from a spring-loaded pistol. Animals were decapitated within 2 sec, brains quickly removed, several brain regions homogenized in cold buffer and radioreceptor assays carried out. There was a 20% increase in binding of [3H]naloxone and [3H]QNB in the hypothalamus but not in the amygdala, striatum, hippocampus, cortex, midbrain and hindbrain. For animals killed 5–10 sec after concussion, when normal conscious behavior had returned, there were no changes in binding between control and concussed rats. Binding of [3H]spiroperidol and [3H]ouabain was unaffected by concussion. To rule out changes due to non-specific stress, one series of rats received a blow to the lower body. There was no change from control in [3H]QNB binding to hypothalamic tissues by a body blow as compared with the blow to the head. The findings suggests decreased binding of endogenous endorphins and acetylcholine to their respective receptors as a result of concussion. The transient deficit in endogenous transmitter binding may temporarily interrupt hypothalamic circuits concerned with the state of consciousness.