ANTISENSE GENE THAT INHIBITS SYNTHESIS OF THE HORMONE ETHYLENE IN TRANSGENIC PLANTS
ANTISENSE GENE THAT INHIBITS SYNTHESIS OF THE HORMONE ETHYLENE IN TRANSGENIC PLANTS
复制标题
抑制转基因植物乙烯激素合成的反义基因
DOI：
10.1038/346284a0
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发表时间：
1990-07-19
期刊：
影响因子：
64.8
通讯作者：
GRIERSON, D
中科院分区：
文献类型：
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作者：
HAMILTON, AJ;LYCETT, GW;GRIERSON, D
乙烯控制高等植物的许多生理和发育过程，包括果实成熟、脱落、衰老和对伤害的反应1。虽然信使RNA在成熟果实和衰老叶片中的积累与乙烯的产生和感知2-4相关，但调控乙烯合成和乙烯刺激基因表达的机制尚不清楚。我们以前已经证明，互补DNA pTOM13对应于一个mRNA，它的合成与成熟果实和受伤叶片中乙烯的合成相关。pTOM13 mRNA编码一个相对分子质量为35,0006的蛋白质。已经对该基因和三个相关的基因组克隆进行了测序，但该蛋白的功能尚不清楚7-9。我们在这里表明，以前只被用来降低已知功能基因10-12表达的反义RNA，当应用于pTOM13时，以一种基因剂量依赖的方式减少乙烯的合成。对这些新突变体的分析表明，pTOM13编码一种多肽，参与乙烯形成酶(ACC-氧化酶)将1-氨基-环丙烷-1-羧酸转化为乙烯。
ETHYLENE controls many physiological and developmental processes in higher plants, including ripening of fruit, abscission, senescence and responses to wounding1. Although the accumulation of messenger RNAs in ripening fruit and senescing leaves has been correlated with ethylene production and perception2–4, the regulatory mechanisms governing ethylene synthesis and the stimulation of gene expression by ethylene are not understood. We have previously shown that the complementary DNA, pTOM13, corresponds to an mRNA whose synthesis is correlated with that of ethylene in ripening fruit and wounded leaves5,6,8. The pTOM13 mRNA encodes a protein of relative molecular mass 35,0006. The cDNA and three related genomic clones have been sequenced, but the function of the protein is unknown7–9. We show here that antisense RNA, which has previously been used only to reduce the expression of genes of known function10–12, when applied to pTOM13, reduces ethylene synthesis in a gene dosage-dependent manner. Analysis of these novel mutants suggests that pTOM13 encodes a polypeptide involved in the conversion of 1-amino-cyclopropane-1-carboxylic acid to ethylene by the ethylene-forming enzyme (ACC-oxidase).