Low-temperature inductively coupled plasma as a method to promote biomineralization on 3D printed poly(lactic acid) scaffolds
Low-temperature inductively coupled plasma as a method to promote biomineralization on 3D printed poly(lactic acid) scaffolds
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DOI：
10.1007/s10853-021-06227-z
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发表时间：
2021-06-14
影响因子：
4.5
通讯作者：
Thomas, Vinoy
中科院分区：
文献类型：
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作者：
Bradford, John P.;Tucker, Bernabe;Thomas, Vinoy
对于骨组织工程，添加剂制造为仿生支架的开发提供了希望；然而，在典型的3D打印方法中使用的常见聚合物往往存在不太理想的表面特性，无法实现适当的矿化。因此，在没有额外修饰的情况下，它们在生物整合方面可能表现不佳。本研究利用低温等离子体对3D打印聚乳酸进行表面改性，以促进体外仿生条件下的矿化。利用傅立叶变换红外光谱(FT-IR)和X射线光电子能谱(XPS)对聚合物表面的磷酸钙进行了检测。用扫描电子显微镜(SEM)对支架表面的矿化产物进行鉴定和成像。此外，表面提取物的荧光分析表明，与对照组相比，处理支架的含钙矿物质沉积水平明显更高。综上所述，这些结果暗示了这种表面修饰方法在骨组织工程应用中的未来潜力。
For bone tissue engineering, additive manufacturing offers promise in the development of biomimetic scaffolds; however, common polymers used in typical 3D printing methods often suffer from less than ideal surface properties for proper mineralization. As a result, they can underperform with respect to biointegration without additional modification. In this study, low-temperature plasma was utilized to modify the surface of 3D printed poly(lactic acid) (PLA) for promoting mineralization in biomimetic in vitro conditions. Detection of calcium phosphates on the polymer surfaces was accomplished via Fourier transform infrared (FT-IR) spectroscopy and X-ray photoelectron (XPS) spectroscopy. Scanning electron microscopy (SEM) was employed to identify and image the mineralization products on the surfaces of the scaffolds. Further, a fluorescent assay of surface extracts identified calcium-containing mineral deposits at significantly higher levels for treated scaffolds versus controls. Taken together, these results suggest the futuristic potential of this surface modification method for bone tissue engineering applications.