Frequencies of PrP gene haplotypes in British sheep flocks and the implications for breeding programmes
Frequencies of PrP gene haplotypes in British sheep flocks and the implications for breeding programmes
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DOI：
10.1111/j.1365-2672.2005.02568.x
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发表时间：
2005-01-01
影响因子：
4
通讯作者：
Hunter, N
中科院分区：
文献类型：
--
作者：
Goldmann, W;Baylis, M;Hunter, N
目的：分析英国绵羊群体中PrP基因单倍型的频率及其与绵羊传染性海绵状脑病(TSE)和绵羊抗TSE育种计划的相关性。方法：从绵羊血液中提取基因组DNA，对PrP基因编码区进行聚合酶链式反应(PCR)扩增并测序。本研究分析了6287个ARQ单倍型中密码子110和245之间的PrP序列，共发现8个变异序列，占所有ARQ单倍型的41%，高于预期。新增的PrP基因二态有M112T、L141F、M137T、H143R、H151C、P168L、Q175E和P241S。结论：ARQ单倍型的发生与羊群瘙痒病的发病无关。与来自英国以外的羊群相比，英国羊群中ARQ单倍型的可变性似乎有所不同。研究的意义和影响：额外的PrP二态可能会影响用于绵羊育种计划中标准PrP基因分型的方法。这些多态中的一些在英国羊群中被发现具有显著的频率，因此在育种计划中应该被考虑。
Aims: To analyse the frequencies of prion (PrP) gene haplotypes in UK sheep flocks and evaluate their relevance to transmissible spongiform encephalopathies (TSEs) and TSE resistance breeding programmes in sheep.Methods and Results: Genomic DNA isolated from sheep blood was PCR amplified for the coding region of the PrP gene and then sequenced. This study has analysed the sequence of PrP between codons 110 and 245 in 6287 ARQ haplotypes revealing a total of eight variant sequences, which represent a higher than expected 41% of all ARQ haplotypes. The additional PrP gene dimorphisms were M112T, L141F, M137T, H143R, H151C, P168L, Q175E and P241S.Conclusion: The results do not suggest a correlation between the occurrence of a specific ARQ haplotype and the scrapie disease status of a flock. The ARQ haplotype variability appears to be different in the UK sheep flocks compared with sheep flocks from outside the UK.Significance and Impact of the Study: Additional PrP dimorphisms may impact on the methodologies used for standard PrP genotyping in sheep breeding programmes. Some of these polymorphisms were found with significant frequencies in the UK sheep flocks and should therefore be considered in breeding programmes.