Evolutionary analysis of mitochondrially encoded proteins of toad-headed lizards, Phrynocephalus, along an altitudinal gradient.
Evolutionary analysis of mitochondrially encoded proteins of toad-headed lizards, Phrynocephalus, along an altitudinal gradient.
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蟾头蜥蜴线粒体编码蛋白沿海拔梯度的进化分析
DOI：
10.1186/s12864-018-4569-1
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发表时间：
2018-03-06
期刊：
影响因子：
4.4
通讯作者：
Brown RP
中科院分区：
文献类型：
--
作者：
Jin Y;Wo Y;Tong H;Song S;Zhang L;Brown RP
生活在高海拔地区的动物必须适应低氧和低温环境，但对潜在的基因变化知之甚少。沙头蜥属的蟾头蜥蜴覆盖着超过4000米的广阔海拔梯度，是研究这种适应反应的有用模型。在第一批考虑选择分布在如此广泛的环境中的外温群的线粒体蛋白编码区的研究中，我们分析了来自所有中国沙棘的19个完整的线粒体基因组(其中8个基因组是首次测序的)。最初的分析使用站点和分支站点模型(程序：PAML)方法来检查非同义词：跨mtDNA树的同义词替换率。发现了10个阳性选择的位点，其中9个与呼吸链酶线粒体复合体I(NADH辅酶Q氧化还原酶)中的ND2、ND3、ND4、ND5和ND6亚基相对应。其中四个地点显示了整个群体的一般长期选择的证据，而其余的地点则显示了跨树的不同分支的间歇性选择的证据。其中一些分支对应于海拔和/或纬度的上升。对蛋白质结构的物理化学变化的分析表明，在被选择的位点上的残基变化对应于主要的功能差异。协同进化的分析指向ND4亚基内选定的位点的协同进化，关键位点与跨线粒体膜的质子转移有关。我们的结果确定线粒体复合体I是这组蜥蜴环境中介选择的目标，这种复合体似乎经常在其他生物中被选择。这使得这些蜥蜴成为未来更详细的分子进化研究的很好候选者。本文的在线版本(10.1186/s12864-0184569-1)包含向授权用户提供的补充材料。
Animals living at high altitude must adapt to environments with hypoxia and low temperatures, but relatively little is known about underlying genetic changes. Toad-headed lizards of the genus Phrynocephalus cover a broad altitudinal gradient of over 4000 m and are useful models for studies of such adaptive responses. In one of the first studies to have considered selection on mitochondrial protein-coding regions in an ectothermic group distributed over such a wide range of environments, we analysed nineteen complete mitochondrial genomes from all Chinese Phrynocephalus (including eight genomes sequenced for the first time). Initial analyses used site and branch-site model (program: PAML) approaches to examine nonsynonymous: synonymous substitution rates across the mtDNA tree. Ten positively selected sites were discovered, nine of which corresponded to subunits ND2, ND3, ND4, ND5, and ND6 within the respiratory chain enzyme mitochondrial Complex I (NADH Coenzyme Q oxidoreductase). Four of these sites showed evidence of general long-term selection across the group while the remainder showed evidence of episodic selection across different branches of the tree. Some of these branches corresponded to increases in altitude and/or latitude. Analyses of physicochemical changes in protein structures revealed that residue changes at sites that were under selection corresponded to major functional differences. Analyses of coevolution point to coevolution of selected sites within the ND4 subunit, with key sites associated with proton translocation across the mitochondrial membrane. Our results identify mitochondrial Complex I as a target for environment-mediated selection in this group of lizards, a complex that frequently appears to be under selection in other organisms. This makes these lizards good candidates for more detailed future studies of molecular evolution. The online version of this article (10.1186/s12864-018-4569-1) contains supplementary material, which is available to authorized users.
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影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
3.7
作者：
Finch TM;Zhao N;Korkin D;Frederick KH;Eggert LS
通讯作者：
Eggert LS
影响因子：
3.5
作者：
Marshall, H. Dawn;Baker, Allan J.;Grant, Allison R.
通讯作者：
Grant, Allison R.
影响因子：
3.7
作者：
Garvin MR;Bielawski JP;Gharrett AJ
通讯作者：
Gharrett AJ
青藏高原沙蜥属生活史特征与海拔相关的变化：它们是否遵循典型的有鳞动物生态地理模式？
影响因子：
2
作者：
Jin, Y. T.;Li, J. Q.;Liu, N. F.
通讯作者：
Liu, N. F.