Ion contents and other properties of isolated cells from dog tracheal epithelium.
Ion contents and other properties of isolated cells from dog tracheal epithelium.
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狗气管上皮分离细胞的离子含量和其他特性。
DOI：
10.1152/ajpcell.1981.241.5.c184
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Highland,E
中科院分区：
文献类型：
--
作者：
Widdicombe,JH;Basbaum,CB;Highland,E
我们已经开发了一种从狗气管粘膜分离细胞的制备方法。完整上皮的三种主要细胞类型（纤毛细胞、分泌细胞和基底细胞）的比例与完整组织中的比例大致相同。这些细胞显示出高活力，如通过排除活体染料、高O2消耗和将氨基酸掺入蛋白质来判断的。细胞内离子含量（mmol/l细胞H2O）为[K]i，150; [Na]i，20;和[Cl]i，50。哇巴因（10（-4）M）引起[Na]i升高和细胞内K的相互损失。
We have developed a preparation of isolated cells from dog tracheal mucosa. The three major cell types of the intact epithelium (ciliated, secretory, and basal) are present in approximately the same proportions as in the intact tissue. These cells show high viability as judged by exclusion of vital dyes, high O2 consumption, and incorporation of amino acids into protein. The intracellular ion contents (in mmol/l cell H2O) are [K]i, 150; [Na]i, 20; and [Cl]i, 50. Ouabain (10(-4) M) causes a rise in [Na]i and a reciprocal loss of intracellular K.