Transcriptional and antagonistic responses of Pseudomonas fluorescens Pf0-1 to phylogenetically different bacterial competitors
Transcriptional and antagonistic responses of Pseudomonas fluorescens Pf0-1 to phylogenetically different bacterial competitors
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DOI：
10.1038/ismej.2010.196
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发表时间：
2011-06-01
期刊：
影响因子：
11
通讯作者：
de Boer, Wietse
中科院分区：
文献类型：
--
作者：
Garbeva, Paolina;Silby, Mark W.;de Boer, Wietse
土壤细菌成功与一系列其他微生物物种竞争的能力对于它们在营养有限的土壤环境中生长和生存至关重要。本研究研究了土壤荧光假单胞菌菌株Pf0-1在营养贫乏的琼脂上与3种系统发育不同的细菌属（芽孢杆菌、Brevundimonas和Pedobacter）对抗的行为和转录反应。这三种细菌属对荧光假单胞菌Pf0-1的密度都有负面影响，因此对营养物质的竞争很明显；这种效应在与芽孢杆菌的相互作用中最为明显。基于微阵列的分析表明，Pf0-1对不同竞争对手的转录反应存在很大差异。荧光假单胞菌Pf0-1对革兰氏阴性菌的基因表达响应与革兰氏阳性菌相比具有较高的相似性。革兰氏阴性菌株也在Pf0-1中引发了一种未知的广谱抗生素的产生。更详细的分析表明，参与信号转导和次生代谢物产生的特定Pf0-1基因的表达受到竞争对手身份的强烈影响，这表明Pf0-1可以区分不同的竞争对手并微调其竞争策略。本文的研究结果表明，荧光假单胞菌Pf0-1对细菌竞争对手表现出一种物种特异性的转录和代谢反应，并为鉴定细菌-细菌相互作用的特定线索、新的竞争策略、抗菌性状和基因提供了新的线索。ISME Journal (2011) 5, 973-985；doi: 10.1038 / ismej.2010.196;主题类别：微生物-微生物和微生物-宿主相互作用
The ability of soil bacteria to successfully compete with a range of other microbial species is crucial for their growth and survival in the nutrient-limited soil environment. In the present work, we studied the behavior and transcriptional responses of soil-inhabiting Pseudomonas fluorescens strain Pf0-1 on nutrient-poor agar to confrontation with strains of three phylogenetically different bacterial genera, that is, Bacillus, Brevundimonas and Pedobacter. Competition for nutrients was apparent as all three bacterial genera had a negative effect on the density of P. fluorescens Pf0-1; this effect was most strong during the interaction with Bacillus. Microarray-based analyses indicated strong differences in the transcriptional responses of Pf0-1 to the different competitors. There was higher similarity in the gene expression response of P. fluorescens Pf0-1 to the Gram-negative bacteria as compared with the Gram-positive strain. The Gram-negative strains did also trigger the production of an unknown broad-spectrum antibiotic in Pf0-1. More detailed analysis indicated that expression of specific Pf0-1 genes involved in signal transduction and secondary metabolite production was strongly affected by the competitors' identity, suggesting that Pf0-1 can distinguish among different competitors and fine-tune its competitive strategies. The results presented here demonstrate that P. fluorescens Pf0-1 shows a species-specific transcriptional and metabolic response to bacterial competitors and provide new leads in the identification of specific cues in bacteria-bacteria interactions and of novel competitive strategies, antimicrobial traits and genes. The ISME Journal (2011) 5, 973-985; doi:10.1038/ismej.2010.196; published online 13 January 2011 Subject Category: microbe-microbe and microbe-host interactions