Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry.
Washing erythrocytes to remove interferents in measurements of zinc protoporphyrin by front-face hematofluorometry.
复制标题
清洗红细胞以去除正面血液荧光法测量原卟啉锌中的干扰物。
作者：
J. Hastka;J. Lasserre;A. Schwarzbeck;M. Strauch;Rüdiger Hehlmann
通过全血荧光测定法测定锌原卟啉 (ZPP)，以检测献血者的铁缺乏情况。在住院患者中，ZPP 与已确定的铁状态标志物无关。我们使用 Aviv 正面血液荧光计对 475 名患者的样本进行了 4500 次 ZPP 测量，并测量了铁蛋白、转铁蛋白饱和度、血红蛋白和红细胞指数。我们发现荧光测定受到血浆中溶解的物质的影响，但可以通过使用洗涤的红细胞消除这种干扰。在验证测试中，日内变化 < 3.5%；每日变化< 6.8%。在 130 名不缺铁的健康人中，ZPP ≤ 40 mumol/mol 血红素，我们认为这是一个正常值。 46 名缺铁患者的平均 ZPP 为 256 (SD 105) mumol/mol 血红素（与铁蛋白的相关性：-0.73；与血红蛋白的相关性：-0.85；P < 0.001）。当使用洗涤的红细胞时，ZPP 的血液荧光测定对于检测健康个体和住院患者的铁缺乏症具有灵敏性和特异性。
Zinc protoporphyrin (ZPP) is determined by hematofluorometry of whole blood to detect iron deficiency in blood donors. In hospitalized patients, ZPP did not correlate with established markers of iron status. We performed 4500 ZPP measurements with the Aviv front-face hematofluorometer in samples from 475 patients and measured ferritin, transferrin saturation, hemoglobin, and erythrocyte indices. We found that the fluorometric determination is affected by substances dissolved in plasma but that this interference can be eliminated by using washed erythrocytes. In validation tests the within-day variation was < 3.5%; the day-to-day variation was < 6.8%. In 130 healthy persons without iron deficiency, ZPP was < or = 40 mumol/mol heme, which we consider a normal value. Mean ZPP in 46 iron-deficient patients was 256 (SD 105) mumol/mol heme (correlation with ferritin: -0.73; with hemoglobin: -0.85; P < 0.001). When washed erythrocytes are used, the hematofluorometric determination of ZPP is sensitive and specific for detecting iron deficiency in otherwise healthy individuals and hospitalized patients.