A novel application of myocardial contrast Echocardiography to evaluate angiogenesis by autologous bone marrow cell transplantation in chronic ischemic pig model
A novel application of myocardial contrast Echocardiography to evaluate angiogenesis by autologous bone marrow cell transplantation in chronic ischemic pig model
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DOI：
10.1016/j.jacc.2003.09.061
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发表时间：
2004-04-07
影响因子：
24
通讯作者：
Kitamura, S
中科院分区：
文献类型：
--
作者：
Fujii, H;Tomita, S;Kitamura, S
目的 我们研究心肌造影超声心动图（MCE）评估骨髓细胞移植后局部灌注的可行性。 背景 细胞移植改善的心肌微血管太小，无法用传统血管造影术观察到。 方法 使用日本生命科学研究所的 14 只小型猪。结扎近端左冠状动脉前降支。一个月后，9头猪活了下来。六头猪接受了左心室前壁自体细胞移植：骨髓单核细胞 (BMMNC) (n = 3) 和骨髓基质细胞 (BMSC) (n = 3)。其他三头猪接受盐水（对照组，n = 3）。一个月后，这些猪被处死。使用 SONOS 5500 系统（飞利浦）测量造影剂的心肌造影强度 (MCI)。在四个区域测量毛细血管密度（CD）和MCI：前间隔（非移植梗塞区域）、前壁（移植梗塞区域）、隔膜（边界区）和侧壁（正常）。我们通过 MCI 和 CD 比较了前间隔和前壁。 结果 在 BMMNC 和 BMSC 亚群中，前壁的 CD 高于前间隔 (p < 0.001)。 MCI 和 CD 之间存在线性关系（声学单位 [AU(2)] = 0.234 CD + 0.010，r = 0.92，p < 0.001）。细胞移植后 1 个月，BMMNC 和 BMSC 亚群的前壁 MCI 增加（p < 0.05），但对照组没有变化。移植梗死区的壁厚比（收缩期/舒张期）大于非移植梗塞区（p < 0.01）。 结论 心肌造影超声心动图可用于评估局部灌注，骨髓细胞移植可增强局部灌注。 (C) 2004 年由美国心脏病学会基金会资助。
OBJECTIVES We investigated the feasibility of myocardial contrast echocardiography (MCE) to evaluate regional perfusion after bone marrow cell transplantation.BACKGROUND The myocardial microvessels improved by cell transplantation are too small to visualize with conventional angiography.METHODS Fourteen mini-pigs from the Nippon Institute for Biological Science were used. The proximal left anterior descending coronary artery was ligated. One month later, nine pigs survived. Six pigs received autologous cell transplantation into the left ventricular anterior wall: bone marrow mononuclear cells (BMMNCs) (n = 3) and bone marrow stromal cells (BMSCs) (n = 3). The other three pigs received saline (control group, n = 3). The pigs were sacrificed one month later. Myocardial contrast intensity (MCI) with a contrast agent was measured using the SONOS 5500 system (Philips). Capillary density (CD) and MCI were measured at four areas: anteroseptum (nontransplanted infarct area), anterior wall (transplanted infarct area), septum (border zone), and lateral wall (normal). We compared the anteroseptum with the anterior wall by MCI and CD.RESULTS In the BMMNC and BMSC subsets, the CD of the anterior wall was higher than that of the anteroseptum (p < 0.001). There was a linear relation between MCI and CD (acoustic unit [AU(2)] = 0.234 CD + 0.010, r = 0.92, p < 0.001). At one month after cell transplantation, MCI of the anterior wall increased in the BMMNC and BMSC subsets (p < 0.05), although it did not change in the control group. The ratio of wall thickness (systole/diastole) in the transplanted infarct area was larger than that in the nontransplanted infarct area (p < 0.01).CONCLUSIONS Myocardial contrast echocardiography is useful to evaluate regional perfusion, which was enhanced by bone marrow cell transplantation. (C) 2004 by the American College of Cardiology Foundation.