LEUKOCYTES ARE REQUIRED FOR INCREASED LUNG MICRO-VASCULAR PERMEABILITY AFTER MICROEMBOLIZATION IN SHEEP
LEUKOCYTES ARE REQUIRED FOR INCREASED LUNG MICRO-VASCULAR PERMEABILITY AFTER MICROEMBOLIZATION IN SHEEP
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DOI：
10.1161/01.res.48.3.344
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发表时间：
1981-01-01
影响因子：
20.1
通讯作者：
STAUB, NC
中科院分区：
文献类型：
--
作者：
FLICK, MR;PEREL, A;STAUB, NC
本文研究了微栓子致不均匀肺动脉阻塞对正常绵羊和白细胞减少绵羊稳态跨血管液体和蛋白质交换的影响。测定肺动脉压、左房压、心输出量、肺淋巴流量和淋巴血浆蛋白浓度。给绵羊动脉注射盐酸甲氯乙胺(0.4 mg/kg，2次)和秋水仙碱(0.1-0.2 mg/kg，仅麻醉绵羊)，造成绵羊严重的白细胞减少。在麻醉绵羊上，经右心房注入直径为200微米的玻璃微珠，使肺血管阻力增加到基线的2-3倍。在循环白细胞水平正常的情况下，绵羊从肺中产生的富含蛋白质的淋巴流量增加，其特征是通透性增加，水肿。对于同等程度的血管阻塞，白细胞减少的绵羊在栓塞术后反应显著减弱。在未麻醉的绵羊中，通过右心房持续注入直径1 mm的气泡，以增加肺血管对.apprx的阻力。基线值的2倍。每只羊都充当自己的对照。在循环白细胞水平正常的情况下，栓塞术期间从肺中流出的富含蛋白质的淋巴增多。当注气结束时，绵羊在24小时内恢复到基线状态。当绵羊严重白细胞减少时，用相同的气量诱导栓子；当阻塞程度相同时，从肺中流出的淋巴和蛋白质明显减少。循环中的白细胞显然是绵羊微血管损伤的关键，微血管损伤导致绵羊肺微栓塞术后肺通透性增加。
The effects of uneven pulmonary artery obstruction by microemboli on steady-state transvascular fluid and protein exchange in normal and leukopenic sheep were studied. Pulmonary artery and left atrial pressures, cardiac output, lung lymph flow and lymph plasma protein concentrations were measured. Sheep were made profoundly leukopenic by administration of intra-arterial mechlorethamine hydrochloride (0.4 mg/kg, 2 doses) and colchicine (0.1-0.2 mg/kg, anesthetized sheep only). In anesthetized sheep, glass beads 200 .mu.m in diameter were injected via the right atrium to raise pulmonary vascular resistance to 2-3 times baseline values. With normal levels of circulating leukocytes, sheep developed an increased protein-rich lymph flow from the lung characteristic of increased permeability edema. Leukopenic sheep had a significantly attenuated response after embolization for equivalent degrees of vascular obstruction. In unanesthetized sheep, air bubbles 1 mm in diameter were continuously infused via the right atrium to raise pulmonary vascular resistance to .apprx. 2 times baseline values. Each sheep served as its own control. With normal circulating leukocyte levels there was an increase in protein-rich lymph flow from the lung during embolization. When the air infusion ended, the sheep recovered to the baseline condition in 24 h. Emboli were induced with the same amount of air when the sheep were profoundly leukopenic; lymph and protein flow from the lung were significantly less for equivalent degrees of obstruction. Circulating leukocytes apparently are essential for the microvascular injury that results in increased permeability in the lungs of sheep after microembolization.