Effects of glucose and biphenyl on aerobic cometabolism of polybrominated diphenyl ethers by Pseudomonas putida: Kinetics and degradation mechanism
Effects of glucose and biphenyl on aerobic cometabolism of polybrominated diphenyl ethers by Pseudomonas putida: Kinetics and degradation mechanism
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DOI：
10.1016/j.ibiod.2015.12.004
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发表时间：
2016-03-01
影响因子：
4.8
通讯作者：
Hu, Yongyou
中科院分区：
文献类型：
--
作者：
Lv, Yuancai;Li, Lianghao;Hu, Yongyou
多溴联苯醚（PBDEs）是一种新兴的持久性有机污染物，由于其极端的持久性和难降解性，其降解仍是一个巨大的挑战。采用恶臭假单胞菌（Pseudomonasputida）对初始浓度为2 mg/L的4种多溴联苯醚（PBDE）同系物（BDE 47、15、3和二苯醚（DE））进行生物修复，并通过动力学分析和代谢机理对PBDE的共代谢进行了研究。生物降解动力学分析”表明，多溴二苯醚与二苯醚上溴取代的数量成反比。葡萄糖（24 mg/L）和联苯（10 mg/L）对PBDEs的生物降解有促进作用。生长动力学分析表明，葡萄糖对PBDEs的消耗具有非竞争性抑制作用，而联苯-PBDEs二元底物体系中既存在竞争性抑制作用，也存在非竞争性抑制作用。并对中间产物进行了GC-MS分析，提出了主要的降解途径。PBDEs降解的主要途径是角向双氧作用，而联苯的加入可以诱导侧向双氧作用。(C)2015爱思唯尔有限公司版权所有。
Polybrominated diphenyl ethers (PBDEs) are emerging persistent organic pollutants and the degradation of PBDEs is still a tremendous challenge owing to their extreme persistence and recalcitrance. In this study, Pseudomonas putida was employed for bioremediation of four PBDE congeners (BDE47, 15, 3 and diphenyl ether (DE)) with an initial concentration of 2 mg/L and the cometabolism of PBDEs was illustrated via kinetics analysis and metabolic mechanism. The biodegradation kinetics analysis" suggested that PBDEs was inversely proportional to the number of bromine substituted on diphenyl ether. Both glucose (24 mg/L) and biphenyl (10 mg/L) played a positive role in promoting the biodegradation of PBDEs. The growth kinetics analysis indicated that glucose had uncompetitive inhibition on PBDEs consumption while both competitive and uncompetitive inhibition existed in biphenyl-PBDEs binary substrate system. Furthermore, the intermediates were identified by GC MS and the main tentative degradation pathways were proposed. The angular dioxygenation was the main pathway for PBDEs degradation, while lateral dioxygenation could be induced when biphenyl was added. (C) 2015 Elsevier Ltd. All rights reserved.