The proton pump inhibitor lansoprazole inhibits rhinovirus infection in cultured human tracheal epithelial cells
The proton pump inhibitor lansoprazole inhibits rhinovirus infection in cultured human tracheal epithelial cells
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DOI：
10.1016/j.ejphar.2004.12.042
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发表时间：
2005-02-21
影响因子：
5
通讯作者：
Sasaki, H
中科院分区：
文献类型：
--
作者：
Sasaki, T;Yamaya, M;Sasaki, H
为了研究质子泵抑制剂兰索拉唑对呼吸道鼻病毒感染的影响，用鼻病毒的一个主要亚型--14型鼻病毒感染人气管上皮细胞。鼻病毒可增加鼻病毒主要受体细胞间黏附分子-1(ICAM-1)的mRNA表达，增加细胞培养上清液中可溶性ICAM-1(sICAM-1)和细胞因子的含量。兰索拉唑可降低鼻病毒上清液的滴度和RNA，降低鼻病毒感染的敏感性，减少ICAM-1mRNA的产生，减少细胞内酸性内含体的数量和荧光强度，降低上清液中sICAM-1和包括IL-1β在内的细胞因子的浓度。白介素1β抗体降低了基线和鼻病毒诱导的ICAM-1的产生。这些结果提示兰索拉唑通过部分内源性IL-1的产生减少ICAM-1，并通过阻止鼻病毒RNA进入内体来抑制鼻病毒感染。兰索拉唑可能通过减少鼻病毒感染时细胞因子和ICAM-1的产生来调节呼吸道炎症。(C)2004爱思唯尔B.V.保留所有权利。
To examine the effects of lansoprazole, a proton pump inhibitor, on rhinovirus infection in airways, human tracheal epithelial cells were infected with a major subgroup of rhmoviruses, type 14 rhinovirus. Rhinovirus increased the mRNA expression of intercellular adhesion molecule-1 (ICAM-1) in the cells, the major rhinovirus receptor, and the content of the soluble form of ICAM- 1 (sICAM-1) and cytokines in supernatants. Lansoprazole reduced supernatant titers and RNA of rhinovirus, the susceptibility to rhinovirus infection, the ICAM-1 mRNA production, the number and fluorescence intensity of acidic endosomes in the cells, and supernatants sICAM-1 and cytokine concentrations including interleukin-1beta. Antibody to interleukin-1beta reduced baseline and rhinovirus-induced ICAM- 1 production. These results suggest that lansoprazole inhibits rhinovirus infection by reducing ICAM-1 via partly endogenous production of interleukin-1, and by blocking the rhinovirus RNA entry into the endosomes. Lansoprazole may modulate airway inflammation by reducing the production of cytokines and ICAM-1 in rhinovirus infection. (C) 2004 Elsevier B.V. All rights reserved.