Therapeutic effects of four strains of probiotics on experimental colitis in mice
Therapeutic effects of four strains of probiotics on experimental colitis in mice
复制标题
DOI：
10.3748/wjg.15.321
复制
发表时间：
2009-01-21
影响因子：
4.3
通讯作者：
Lu, Fang-Gen
中科院分区：
文献类型：
--
作者：
Chen, Lin-Lin;Wang, Xue-Hong;Lu, Fang-Gen
目的：探讨4种益生菌（粪乳杆菌、嗜酸乳杆菌、丁酸乳杆菌和青春期乳杆菌）对葡聚糖硫酸钠（DSS）诱导的Balb/ C小鼠实验性结肠炎的治疗作用。方法：80只Balb/c小鼠随机分为8组。每天记录体重减轻、粪便特征、粪便隐血及便血情况。同时每日评估疾病活动指数（DAI）评分。第13天测量结肠长度并进行组织学评分。检测髓过氧化物酶（MPO）活性。采用ELISA和RT-PCR检测白细胞介素-1 （IL-1）和IL-4的表达。结果：4株益生菌均能减轻dss诱导的实验性结肠炎小鼠的炎症状况。所有服用益生菌的小鼠体重减轻的速度都有所减缓。在益生菌治疗结束时，甚至可以观察到体重增加。益生菌组小鼠DAI和组织学评分均低于对照组（粪肠杆菌组为1.9 +/- 0.2比8.6 +/- 0.4,P < 0.05）。益生菌组小鼠结肠长度明显长于对照组（10.3 +/- 0.34 vs 8.65 +/- 0.77，粪肠杆菌组P < 0.05）。4株益生菌均降低了MP活性和IL-1的表达，提高了IL-4的表达。粪肠杆菌对dss诱导小鼠实验性结肠炎的作用优于其他3种菌株。结论：4株益生菌对实验性结肠炎小鼠有良好的治疗作用。粪肠杆菌对小鼠dss性实验性结肠炎的治疗效果优于其他三种菌株。补充益生菌为UC提供了新的治疗方法。(C) 2009 WJG出版社和白石登出版社。版权所有。
AIM: To investigate the therapeutic effects of four strains of probiotics (E. feacalis, L. acidophilus, C butyricum and B. adolescentis) on dextran sulphate sodium (DSS)-induced experimental colitis in Balb/c mice.METHODS: Eighty Balb/c mice were randomly divided into 8 groups. Weight-loss, fecal character, fecal occult blood and hematochezia were recorded daily. Disease activity index (DAI) scores were also evaluated everyday. Length of colon was measured and histological scores were evaluated on the 13th day. Myeloperoxidase (MPO) activity was detected. Interleukin-1 (IL-1) and IL-4 expression was detected by ELISA and RT-PCR.RESULTS: The four strains of probiotics relieved the inflammatory condition of DSS-induced experimental colitis in mice. Weight loss was slowed down in all probiotics-treated mice. Even weight gain was observed by the end of probiotics treatment. The DAI and histological scores of probiotics-treated mice were lower than those of mice in the control group (1.9 +/- 0.2 vs 8.6 +/- 0.4, P < 0.05 for E. faecalis). The length of colon of probiotics-treated mice was longer than that of mice in the control group (10.3 +/- 0.34 vs 8.65 +/- 0.77, P < 0.05 for E faecalis). The four strains of probiotics decreased the MP activity and the IL-1 expression, but increased the IL-4 expression. E faecalis had a better effect on DSS-induced experimental colitis in mice than the other three strains.CONCLUSION: The four strains of probiotics have beneficial effects on experimental colitis in mice. E. faecalis has a better effect on DSS-incluced experimental colitis in mice than the other three strains. Supplement of probiotics provides a new therapy for UC. (C) 2009 The WJG Press and Baishideng. All rights reserved.