Noninvasive assessment of angiogenesis by ultrasound and microbubbles targeted to αv-integrins
Noninvasive assessment of angiogenesis by ultrasound and microbubbles targeted to αv-integrins
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DOI：
10.1161/01.cir.0000044916.05919.8b
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发表时间：
2003-01-28
期刊：
影响因子：
37.8
通讯作者：
Lindner, JR
中科院分区：
文献类型：
--
作者：
Leong-Poi, H;Christiansen, J;Lindner, JR
背景-目前缺乏表征血管生成过程中新血管形成的非侵入性方法。我们假设，血管生成可以成像与使用对比增强超声（CEU）与微泡靶向α（V）-integrin.Methods和Results-Microbubbles靶向α（V）-integrin制备通过共轭echistatin（MBE）或单克隆抗体对鼠α（V）（MBalpha）的表面。还制备了对照微泡（MBc）。这些微泡的微血管行为通过用缓释FGF-2治疗4天的小鼠的提睾肌的活体显微镜来评估。微血管潴留更大（P
Background-Noninvasive methods for characterizing neovessel formation during angiogenesis are currently lacking. We hypothesized that angiogenesis could be imaged with the use of contrast-enhanced ultrasound (CEU) with microbubbles targeted to alpha(v)-integrins.Methods and Results-Microbubbles targeted to alpha(v)-integrins were prepared by conjugating echistatin (MBE) or monoclonal antibody against murine alpha(v) (MBalpha) to their surface. Control microbubbles (MBc) were also prepared. The microvascular behavior of these microbubbles was assessed by intravital microscopy of the cremaster muscle in mice treated for 4 days with sustained-release FGF-2. Microvascular retention was much greater (P