Functional study of endothelin B receptors in satellite glial cells in trigeminal ganglia
Functional study of endothelin B receptors in satellite glial cells in trigeminal ganglia
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DOI：
10.1097/wnr.0b013e3283472487
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发表时间：
2011-07-13
期刊：
影响因子：
1.7
通讯作者：
Hanani, Menachem
中科院分区：
文献类型：
--
作者：
Feldman-Goriachnik, Rachel;Hanani, Menachem
内皮素（ET）受体在感觉神经节的卫星胶质细胞中有免疫组织化学证据，但没有关于这些受体功能的信息。我们使用钙成像来研究这个问题，在分离的小鼠三叉神经节，发现卫星神经胶质细胞是高度敏感的ET-1，阈值为0.05 nM。在ET-1浓度超过1 nM时，反应显示出强烈的脱敏作用。一个大的组成部分的反应持续时，钙离子从外部介质中删除，从内部存储与钙离子释放一致。受体选择性试剂的使用表明，反应是由ETB受体介导的。我们的结论是，卫星胶质细胞显示内皮素受体，这可能参与三叉神经节神经元胶质细胞的通信。NeuroReport 22：465-469（C）2011年威科健康垂直酒吧Lippincott威廉姆斯&威尔金斯。
There is immunohistochemical evidence for endothelin ( ET) receptors in satellite glial cells in sensory ganglia, but there is no information on the function of these receptors. We used calcium imaging to study this question in isolated mouse trigeminal ganglia and found that satellite glial cells are highly sensitive to ET-1, with threshold at 0.05 nM. Responses displayed strong desensitization at ET-1 concentrations of more than 1 nM. A large component of the response persisted when Ca2+ was deleted from the external medium, consistent with Ca2+ release from internal stores. The use of receptor selective agents showed that the responses were mediated by ETB receptors. We conclude that satellite glial cells display endothelin receptors, which may participate in neuron-glia communications in the trigeminal ganglia. NeuroReport 22:465-469 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.