NEUROTENSIN IN THE LATERAL HYPOTHALAMIC AREA - ORIGIN AND FUNCTION
NEUROTENSIN IN THE LATERAL HYPOTHALAMIC AREA - ORIGIN AND FUNCTION
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DOI：
10.1016/0306-4522(95)00261-g
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发表时间：
1995-11-01
期刊：
影响因子：
3.3
通讯作者：
CECHETTO, DF
中科院分区：
文献类型：
--
作者：
ALLEN, GV;CECHETTO, DF
应用荧光逆行追踪和神经降压素样免疫反应研究了下丘脑外侧区神经降压素的起源。FluoroGold荧光逆行追踪结合神经降压素免疫组织化学方法，在大鼠杏仁核中央核、穹窿周围区和臂旁核内发现了大量向下丘脑后外侧区投射的神经降压素免疫反应神经元。在终纹床核、隔外侧核、视前内侧区、下丘脑室旁核和室旁核、下丘脑前外侧区和中缝背核中，有少数神经降压素阳性神经元投射到下丘脑外侧区，并探讨了神经降压素在下丘脑背核自主神经调节输入中的作用。调查下丘脑外侧区的单个单位响应于岛叶皮层中的位点的电刺激（500-900 μ A，0.5 Hz），从该位点可以引起心血管加压或降压反应。这些单位进行了测试的影响，神经降压素对刺激的岛叶皮层的反应。在60个自发放电的神经元中，有27个单位对电刺激心血管部位有反应。在对心血管部位的刺激有反应的单位中，14个单位仅表现出兴奋，10个单位表现出兴奋后抑制，3个单位表现出抑制。0.1-1.0 mM神经降压素（25-100 nA，pH 5.0-6.0）的离子导入增强了6种兴奋性反应，对抑制性反应没有影响。此外，9个神经元表现出增加的自发活动与神经降压素的离子导入。在这些神经元中，有3个被岛叶刺激所兴奋，6个没有反应，这些发现表明神经降压素可能起源于下丘脑外侧区，并表明神经降压素在岛叶皮层的心血管调节输入的调制中起作用。
The origin of neurotensin in the lateral hypothalamus was investigated by means of fluorescent retrograde tract tracing and neurotensin-like immunoreactivity. Following fluorescent retrograde tract tracing with FluoroGold combined with neurotensin immunohistochemistry in the rat brain, numerous neurotensin-immunoreactive neurons with projections to the posterior lateral hypothalamic area were identified in the central nucleus of the amygdala, perifornical area and the parabrachial nucleus. Fewer numbers of neurotensin-positive neurons with projections to the lateral hypothalamic area were observed in the bed nucleus of the stria terminalis, lateral septal nucleus, medial preoptic area, peri- and paraventricular nuclei of the hypothalamus, anterior lateral hypothalamic area and dorsal raphe nucleus.In addition, the role of neurotensin in the modulation of autonomic regulatory input from the insula was investigated. The lateral hypothalamic area was surveyed for single units responding to electrical stimulation (500-900 mu A, 0.5 Hz) of sites in the insular cortex from which cardiovascular presser or depressor responses could be elicited. These units were tested for the influence of neurotensin on responses to stimulation of the insular cortex. Of 60 spontaneously firing neurons, 27 units responded to electrical stimulation of cardiovascular sites in the insula. Of the units responding to stimulation of cardiovascular sites in the insula, 14 units showed excitation only, 10 units showed excitation followed by inhibition and three units showed inhibition. Iontophoresis of 0.1-1.0 mM neurotensin (25-100 nA, pH 5.0-6.0) potentiated six of the excitatory responses and showed no effect on the inhibitory responses. In addition, nine neurons showed an increase in spontaneous activity with iontophoresis of neurotensin. Of these neurons, three were excited by insular stimulation and six did not respond.These findings indicate the likely origin of neurotensin in the lateral hypothalamic area and demonstrate that neurotensin has a role in the modulation of some of the cardiovascular regulatory input from the insular cortex.