Rearrangement of mitochondrial tRNA genes in flat bugs (Hemiptera: Aradidae).
Rearrangement of mitochondrial tRNA genes in flat bugs (Hemiptera: Aradidae).
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扁虫线粒体 tRNA 基因的重排（半翅目：Aradidae）
DOI：
10.1038/srep25725
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发表时间：
2016-05-16
影响因子：
4.6
通讯作者：
Cai W
中科院分区：
文献类型：
--
作者：
Song F;Li H;Shao R;Shi A;Bai X;Zheng X;Heiss E;Cai W
在异翅目五胚亚目中发现了典型的昆虫线粒体（mt）基因组组织，即一条染色体包含37个基因。这些真虫的mt基因排列通常与昆虫祖先的mt基因排列相同。然而，在扁虫的两个亚科（Mezirinae和Calisiinae， Aradidae家族）中发现了转移RNA （tRNA）基因的重排。在这项研究中，我们对来自其他三个亚科（Aradinae， Carventinae和动脉瘤科）的四个物种的全基因组进行了测序。我们在这四个物种中都发现了tRNA基因重排。所有重排的tRNA基因都位于线粒体控制区和cox1之间，表明该区域是扁虫基因重排的热点区域；重排可能是由串联重复和基因的随机缺失事件引起的。此外，我们的系统发育和年代分析表明，在迄今为止所研究的5个扁虫亚科的最近共同祖先中，nqandtrni的位置互换发生在1.62亿年前，而ncandtrna的位置互换发生在后来形成Calisiinae的谱系中，而ncandtrna的位置互换发生在后来形成Aradinae的谱系中，而ncandtrna的位置互换发生在134mya之后。
The typical insect mitochondrial (mt) genome organization, which contains a single chromosome with 37 genes, was found in the infraorder Pentatomomorpha (suborder Heteroptera). The arrangement of mt genes in these true bugs is usually the same as the ancestral mt gene arrangement of insects. Rearrangement of transfer RNA (tRNA) genes, however, has been found in two subfamilies of flat bugs (Mezirinae and Calisiinae, family Aradidae). In this study, we sequenced the complete mt genomes of four species from three other subfamilies (Aradinae, Carventinae and Aneurinae). We found tRNA gene rearrangement in all of these four species. All of the rearranged tRNA genes are located between the mitochondrial control region andcox1, indicating this region as a hotspot for gene rearrangement in flat bugs; the rearrangement is likely caused by events of tandem duplication and random deletion of genes. Furthermore, our phylogenetic and dating analyses indicated that the swap of positions betweentrnQandtrnIoccurred ~162 million years ago (MYA) in the most recent common ancestor of the five subfamilies of flat bugs investigated to date, whereas the swap of positions betweentrnCandtrnWoccurred later in the lineage leading to Calisiinae, and the translocation oftrnCandtrnYoccurred later than 134 MYA in the lineage leading to Aradinae.