ABSCISIC-ACID ACCUMULATION IN LEAVES AND CULTURED-CELLS DURING HEAT ACCLIMATION IN GRAPES
ABSCISIC-ACID ACCUMULATION IN LEAVES AND CULTURED-CELLS DURING HEAT ACCLIMATION IN GRAPES
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DOI：
10.21273/hortsci.28.1.50
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发表时间：
1993-01-01
期刊：
影响因子：
1.9
通讯作者：
RAJASHEKAR, CB
中科院分区：
文献类型：
--
作者：
ABASS, M;RAJASHEKAR, CB
研究了葡萄（Vitis spp.， Venus和Veeblanc）叶片和培养细胞的耐热性和内源ABA水平。植物和培养细胞分别在38℃和36℃环境下驯化。在驯化温度下，植物或细胞的耐热性迅速增加，叶片和培养细胞的耐热性分别在10 ~ 16小时和10 ~ 12小时达到最大值。在热驯化的第一个小时，在叶片和培养细胞达到最大耐热性之前，游离和结合ABA水平急剧上升。热驯化过程中ABA含量的增加是未驯化对照的2 ~ 3倍，ABA在组织中积累高峰的时间与叶片和培养细胞的最大耐热性基本一致。外源ABA诱导培养细胞耐热性。添加7.6和9.5 gm的ABA后，24 h的耐热性显著提高，表明ABA可能是葡萄高温驯化和耐热诱导的一个因素。化学名称：脱落酸（ABA）。
Heat tolerance and endogenous ABA levels in leaves and cultured grape cells (Vitis spp., cultivars Venus and Veeblanc) were evaluated during heat acclimation. Plants and cultured cells were acclimated at 38 and 36C, respectively. Heat tolerance increased rapidly after exposing plants or cells to acclimation temperatures, reaching a maximum after 10 to 16 hours and 10 to 12 hours for leaves and cultured cells, respectively. Free and bound ABA levels increased sharply during the first hour of heat acclimation, before leaves and cultured cells reached their maximum heat tolerance. The increase in ABA during heat acclimation was 2- to 3-fold that of the nonacclimated control, and the time of the ABA accumulation peak in tissue roughly corresponded to the maximum heat tolerance in leaves and cultured cells. Heat tolerance was induced in cultured cells by exogenous ABA application. Heat tolerance increased significantly after 24 hours of ABA application at 7.6 or 9.5 gm. The results suggest that ABA may be a factor in high-temperature acclimation and heat-tolerance induction in grapes. Chemical name used: abscisic acid (ABA).