Selection and characterization of a new switchgrass (Panicum virgatum L.) line with high somatic embryogenic capacity for genetic transformation.
Selection and characterization of a new switchgrass (Panicum virgatum L.) line with high somatic embryogenic capacity for genetic transformation.
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DOI：
10.1016/j.scienta.2011.05.016
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发表时间：
2011-07
影响因子：
4.3
通讯作者：
Bin Xu;Lin-Kai Huang;Zhengxing Shen;G. Welbaum;Xunzhong Zhang;Bingyu Zhao
中科院分区：
文献类型：
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作者：
Bin Xu;Lin-Kai Huang;Zhengxing Shen;G. Welbaum;Xunzhong Zhang;Bingyu Zhao
柳枝稷在园艺上用作观赏植物，在农学上用作动物饲料和假定的生物能源作物。利用柳枝稷成熟种子产生的体细胞胚性愈伤组织进行遗传转化是柳枝稷改良性状的一种策略。本研究采用组织培养轮回选择法，从柳枝稷优良品系中选育出一个上级柳枝稷新品系HR8。阿拉莫HR8种子收获后发芽率为82%，而Alamo种子为26.8%。HR8种子萌发产生84.9%的SE愈伤组织。HR8种子具有较高的内源脱落酸（阿坝）含量，并在培养中对外源阿坝的添加有不同的反应。从柳枝稷种子和愈伤组织中分离内生菌。HR8愈伤组织的内生菌污染比‘Alamo’愈伤组织少。HR8 SE愈伤组织可使用农杆菌进行遗传转化。因此，HR 8是一个产生SE愈伤组织和农杆菌介导转化的优良上级系。
Switchgrass (Panicum virgatum L.) is used horticulturally as an ornamental and agronomically as an animal feedstock and a putative bio-energy crop. Genetic transformation, using somatic embryogenic (SE) callus derived from mature seeds, is one strategy for improving switchgrass traits. A superior switchgrass line, HR8, was developed in this study using recurrent tissue culture selection from cv. Alamo. Eighty two percent of HR8 seeds germinated after harvest comparing to 26.8% for unselected ‘Alamo’. HR8 seeds that germinated produced 84.9% SE callus. HR8 seeds had higher endogenous abscisic acid (ABA) contents and responded differently to exogenous additions of ABA in culture. Endophytes were isolated from switchgrass seeds and callus. HR8 callus had less endophytic contamination than that of ‘Alamo’ callus. HR8 SE calli were genetically transformable using Agrobacterium. Therefore, HR8 is a superior line for generating SE callus and Agrobacterium-mediated transformation.