Development and cytolytic function of intestinal intraepithelial T lymphocytes in antigen-minimized mice
Development and cytolytic function of intestinal intraepithelial T lymphocytes in antigen-minimized mice
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DOI：
10.1046/j.1365-2567.1996.d01-740.x
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发表时间：
1996-10-01
期刊：
影响因子：
6.4
通讯作者：
Ohwaki, M
中科院分区：
文献类型：
--
作者：
KawaguchiMiyashita, M;Shimizu, K;Ohwaki, M
小肠中的上皮内T淋巴细胞（IEL）由携带α β T-tell受体（TCR）的T细胞（α β-IEL）和携带γ δ TCR的T细胞（γ δ-IEL）组成。在喂食天然饮食（Nat-GF）的无菌（GF）小鼠中，α β-IEL的发育和细胞溶解活化急剧减弱，但γ δ-IEL的数量和细胞毒性在常规（CV）和Nat-GF小鼠之间相当。我们比较了喂食C57 BL/6品系抗原最小化饮食的Nat-GF小鼠和GF小鼠（AgM-GF小鼠）的IEL特性，以评估肠道抗原负荷对IEL发展的影响。AgM-GF小鼠的α-β-IEL和γ-δ-IEL数量分别比Nat-GF小鼠少1.9倍和1.4倍。与CV小鼠相比，Nat-Gf小鼠的α β-IEL中CD 4（+）8（-）、CD 4（-）8 α β（+）和CD 4（+）8（+）亚群比例显著降低，导致CD 4（-）8 α α（+）亚群比例升高，但Nat-GF和AgM-GF小鼠的α β-IEL亚群构成相似。相比之下，CV之间γ δ-IEL的相对组成没有差异。Nat-GF和AgM-GF小鼠中，α β-IEL显示低的细胞溶解活性，在抗原最小化的γ δ-IEL下几乎被剥夺了它们的细胞毒性，Nat-GF小鼠中，它们的细胞溶解活性在AgM-GF小鼠中显著降低。这些结果表明，γ-δ-IEL的激活与胃肠道中的微生物定植无关，但其激活是对除活微生物外的外源性抗原物质的响应。
Intraepithelial T lymphocytes in the small intestine (IEL) consist of alpha beta T-tell receptor (TCR)-bearing T cells (alpha beta-IEL) and gamma delta TCR-bearing T cells (gamma delta-IEL). Development and cytolytic activation uf alpha beta-IEL sharply attenuate in germ-free (GF) mice fed a natural diet (Nat-GF), but the number and cytotoxicity of gamma delta-IEL are comparable between conventional(CV) and Nat-GF mice, In this report. we compared the properties of IEL in Nat-GF mice and GF mice fed antigen-minimized diet (AgM-GF mice) of C57BL/6 strain to evaluate an influence of gut antigenic load on IEL development. Numbers of alpha beta-IEL and gamma delta-IEL in AgM-GF mice were less by 1.9- and 1.4-fold than those in Nat-GF mice, respectively. Significant decreases in the proportions of CD4(+)8(-), CD4(-)8 alpha beta(+), and CD4(+)8(+) subsets and a resultant increase in the ratio of CD4(-)8 alpha alpha(+) subset were evident in alpha beta-IEL of Nat-Gf mice compared with CV mice, but the subset constitution of alpha beta-IEL was similar between Nat-GF and AgM-GF mice. In contrast, relative composition of gamma delta-IEL was not different between CV. Nat-GF, and AgM-GF mice, alpha beta-IEL displayed low cytolytic activity in were almost deprived of their cytotoxicity under the antigen-minimized gamma delta-IEL were strongly cytolytic ill Nat-GF mice their cytolytic activity was remarkably reduced in AgM-GF mice. These results indicate that gamma delta-IEL are activated independently of microbial colonization in the gastrointestinal tract but their activation occurs in response to the exogenous antigenic substances other than live micro-organisms.