Trimethylamine N-Oxide Does Not Impact Viability, ROS Production, and Mitochondrial Membrane Potential of Adult Rat Cardiomyocytes
Trimethylamine N-Oxide Does Not Impact Viability, ROS Production, and Mitochondrial Membrane Potential of Adult Rat Cardiomyocytes
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DOI：
10.3390/ijms20123045
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发表时间：
2019-06-02
影响因子：
5.6
通讯作者：
Gallo, Maria Pia
中科院分区：
文献类型：
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作者：
Querio, Giulia;Antoniotti, Susanna;Gallo, Maria Pia
氧化三甲胺（Trimethylamine N-oxide，TMAO）是一种有机化合物，来源于食物中的胆碱和左旋肉碱。它作为渗透剂、蛋白质稳定剂和电子受体，在不同动物中表现出不同的生物学功能。最近的研究指出，在人类中，高循环水平的TMAO与动脉粥样硬化和其他心血管疾病的进展有关。然而，关于TMAO在心肌细胞参数中的直接作用的研究仍然有限。本工作的目的是研究TMAO对离体成年大鼠心肌细胞的作用。TMAO在100 μ M和10 mM的浓度，从1至24小时的治疗，不影响细胞活力，肌节长度，细胞内ROS，和线粒体膜电位。此外，同时用TMAO和已知的心脏损伤（例如H2 O2或阿霉素）治疗不影响治疗效果。总之，TMAO不能被认为是急性情况下细胞水平心脏损伤的直接原因或加剧风险因素。
Trimethylamine N-oxide (TMAO) is an organic compound derived from dietary choline and L-carnitine. It behaves as an osmolyte, a protein stabilizer, and an electron acceptor, showing different biological functions in different animals. Recent works point out that, in humans, high circulating levels of TMAO are related to the progression of atherosclerosis and other cardiovascular diseases. However, studies on a direct role of TMAO in cardiomyocyte parameters are still limited. The purpose of this work is to study the effects of TMAO on isolated adult rat cardiomyocytes. TMAO in both 100 mu M and 10 mM concentrations, from 1 to 24 h of treatment, does not affect cell viability, sarcomere length, intracellular ROS, and mitochondrial membrane potential. Furthermore, the simultaneous treatment with TMAO and known cardiac insults, such as H2O2 or doxorubicin, does not affect the treatment's effect. In conclusion, TMAO cannot be considered a direct cause or an exacerbating risk factor of cardiac damage at the cellular level in acute conditions.