Regulation of ryanodine receptor-mediated Ca(2+) release in vas deferens smooth muscle cells.
Regulation of ryanodine receptor-mediated Ca(2+) release in vas deferens smooth muscle cells.
复制标题
兰尼碱受体介导的输精管平滑肌细胞 Ca(2) 释放的调节。
DOI：
10.1254/jphs.09037fp
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发表时间：
2009
影响因子：
3.5
通讯作者：
Y. Imaizumi
中科院分区：
文献类型：
--
作者：
Akitoshi Ohno;S. Ohya;H. Yamamura;Y. Imaizumi
在输精管（VD）平滑肌细胞（SMCs）上，观察了Ryanodine受体（RyR）介导的Ca（2+）释放及雄激素受体（AR）拮抗剂氟替卡松对其的调节作用。VD和VDSMC获自经口给药的两组雄性大鼠。100 mg/kg流感病毒（Flu）或溶媒（Vehicle）。无论是自发的还是咖啡因诱导的Ca（2+）释放，Flu组的单个VDSMCs的Ca（2+）释放量都明显小于赋形剂组。有趣的是，100 μ M去甲肾上腺素使Flu组的VDSMCs [Ca（2+）]（i）升高的幅度大于赋形剂组。在来自流感病毒的组织制备物中，通过直接电刺激诱导的收缩显示出对30 μ M ryanodine的敏感性低于来自载体的那些。实时荧光定量PCR分析显示，RyR 2和RyR 3在VD中表达，而在Flu中表达明显减少。氟替卡松处理后，总RyR蛋白表达显著降低，而1，4，5-三磷酸肌醇受体（IP 3R）蛋白表达无明显变化。结果表明，长期阻断AR可降低RyR的表达及其对血管收缩的作用，但对IP 3R的作用无明显影响。
Ca(2+) release from intracellular store sites via the ryanodine receptor (RyR) and hormonal regulation by flutamide, an androgen-receptor (AR) antagonist, on it were examined in vas deferens (VD) smooth muscle cells (SMCs). VD and VDSMCs were obtained from two groups of male rats that were treated p.o. with 100 mg/kg flutamide (Flu) or vehicle (Vehicle). Both spontaneous and caffeine-induced Ca(2+) releases were markedly smaller in single VDSMCs from Flu than in those from Vehicle. Interestingly, [Ca(2+)](i) rise by 100 muM norepinephrine in VDSMCs from Flu was larger than that in those from Vehicle. The contractions induced by direct electrical stimulation in tissue preparations from Flu showed lower susceptibility to 30 muM ryanodine than those from Vehicle. Real-time PCR analyses revealed that the transcripts of ryanodine receptor (RyR) type 2 and type 3 (RyR2 and RyR3) were expressed in VD and markedly reduced in Flu. The protein expression of total RyR was significantly reduced by flutamide treatment, but that of inositol 1,4,5-trisphosphate receptor (IP3R) was not affected. It can be strongly suggested that long term block of AR by flutamide reduced the expression of RyR and its contribution to the contraction, but not those of IP3R in VDSMCs.
影响因子：
15.9
作者：
Lehnart, Stephan E.;Mongillo, Marco;Marks, Andrew R.
通讯作者：
Marks, Andrew R.