Structural Evidence of Glycoprotein Assembly in Cellular Membrane Compartments prior to Alphavirus Budding
Structural Evidence of Glycoprotein Assembly in Cellular Membrane Compartments prior to Alphavirus Budding
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DOI：
10.1128/jvi.00036-10
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发表时间：
2010-11-01
影响因子：
5.4
通讯作者：
Cheng, R. Holland
中科院分区：
文献类型：
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作者：
Soonsawad, Pan;Xing, Li;Cheng, R. Holland
甲病毒的膜糖蛋白在子代病毒粒子的组装和出芽中起关键作用。然而，关于病毒糖蛋白转运到质膜的知识是模糊的。在这项研究中，我们调查的作用，细胞病变空泡II型（CPV-II）通过原位电子断层扫描的甲病毒感染的细胞。结果表明，CPV-Ⅱ含有的病毒糖蛋白排列在螺旋管状阵列类似的基本组织的糖蛋白三聚体的包膜上的成熟病毒粒子。CPV-II的位置邻近病毒出芽的网站表明一个模型的运输结构组件的网站出芽。因此，CPV-II的结构特征可用于评估用于复制子生产的包装细胞系的设计。
Membrane glycoproteins of alphavirus play a critical role in the assembly and budding of progeny virions. However, knowledge regarding transport of viral glycoproteins to the plasma membrane is obscure. In this study, we investigated the role of cytopathic vacuole type II (CPV-II) through in situ electron tomography of alphavirus-infected cells. The results revealed that CPV-II contains viral glycoproteins arranged in helical tubular arrays resembling the basic organization of glycoprotein trimers on the envelope of the mature virions. The location of CPV-II adjacent to the site of viral budding suggests a model for the transport of structural components to the site of budding. Thus, the structural characteristics of CPV-II can be used in evaluating the design of a packaging cell line for replicon production.