GenTHREADER: An efficient and reliable protein fold recognition method for genomic sequences
GenTHREADER: An efficient and reliable protein fold recognition method for genomic sequences
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DOI：
10.1006/jmbi.1999.2583
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发表时间：
1999-04-09
影响因子：
5.6
通讯作者：
Jones, DT
中科院分区：
文献类型：
--
作者：
Jones, DT
提出了一种快速、可靠的蛋白质折叠识别方法。该方法使用传统的序列比对算法生成比对，然后通过来自线程技术的方法进行评估。作为最后一步，每个线程模型由神经网络进行评估，以便在所提出的预测中产生单个置信度。该方法的速度，沿着其灵敏度和非常低的假阳性率，使其成为自动预测翻译的细菌基因组（蛋白质组）中所有蛋白质结构的理想方法。该方法已被应用于生殖支原体的基因组，结果分析表明，多达46%的蛋白质来源于预测的蛋白质编码区具有显着的关系，已知结构的蛋白质。然而，在某些情况下，只能预测蛋白质的一个结构域，当计算为整个蛋白质组中氨基酸残基数目的分数时，总覆盖率为30%。(C)北京：科学出版社.
A new protein fold recognition method is described which is both fast and reliable. The method uses a traditional sequence alignment algorithm to generate alignments which are then evaluated by a method derived from threading techniques. As a final step, each threaded model is evaluated by a neural network in order to produce a single measure of confidence in the proposed prediction. The speed of the method, along with its sensitivity and very low false-positive rate makes it ideal for automatically predicting the structure of all the proteins in a translated bacterial genome (proteome). The method has been applied to the genome of Mycoplasma genitalium, and analysis of the results shows that as many as 46% of the proteins derived from the predicted protein coding regions have a significant relationship to a protein of known structure. Ln some cases, however, only one domain of the protein can be predicted, giving a total coverage of 30 % when calculated as a fraction of the number of amino acid residues in the whole proteome. (C) 1999 Academic Press.