Human blood monocytes interact with type I collagen through αxß2 integrin (CD11c-CD18, gp150-95)
Human blood monocytes interact with type I collagen through αxß2 integrin (CD11c-CD18, gp150-95)
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DOI：
10.4049/jimmunol.164.11.5928
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发表时间：
2000-06-01
影响因子：
4.4
通讯作者：
Gillery, P
中科院分区：
文献类型：
--
作者：
Garnotel, R;Rittié, L;Gillery, P
人血单核细胞在炎症和伤口愈合的早期阶段被吸引到结缔组织中，并与驻留细胞和细胞外基质蛋白局部相互作用。本实验以人外周血单核细胞和鼠尾腱I型胶原为材料，研究了I型胶原对单核细胞粘附和超氧阴离子产生的影响。酸溶性和胃蛋白酶消化的I型胶原均能促进单核细胞粘附，而只有端肽完整的酸溶性胶原才能诱导超氧阴离子产生，单核细胞在酸溶性I型胶原上的粘附和活化依赖于二价阳离子的存在，针对整合素亚基CD 11 c和CD 18的mAb特异性地抑制单核细胞在I型胶原上的粘附和活化。将从单核细胞获得的蛋白膜提取物在胶原I-Sepharose 4 B上进行亲和层析，并使用特异性抗整联蛋白亚基Ab通过Western印迹进行分析。在酸溶性和胃蛋白酶消化的胶原的情况下，用抗CD 11 c和CD 18整联蛋白亚基的mAb显示两条带，我们的研究结果表明，单核细胞与I型胶原通过CD 11 c-CD 18（α（x）β（2））整合素相互作用，促进其粘附和活化，对于单核细胞活化，I型胶原蛋白端肽的特定结构域是必需的。单核细胞和胶原蛋白之间的相互作用最有可能参与表征炎症初始阶段的级联事件。
Human blood monocytes are attracted into connective tissues during early steps of inflammation and wound healing, and locally interact with resident cells and extracellular matrix proteins. We studied the effects of type I collagen on monocyte adhesion and superoxide anion production, using human monocytes elutriated from peripheral blood and type I collagen obtained from rat tail tendon, Both acid-soluble and pepsin-digested type I collagens promoted the adhesion of monocytes, whereas only acid-soluble collagen with intact telopeptides induced the production of superoxide, Adhesion and activation of monocytes on acid-soluble type I collagen depended on the presence of divalent cations, mAbs directed against integrin subunits CD11c and CD18 specifically inhibited adhesion and activation of monocytes on type I collagen. Protein membrane extracts obtained from monocytes were submitted to affinity chromatography on collagen I-Sepharose 4B, and analyzed by Western blotting using specific anti-integrin subunit Abs, In the case of both acid-soluble and pepsin-digested collagens, two bands were revealed with mAbs against CD11c and CD18 integrin subunits, Our results demonstrate that monocytes interact with type I collagen through CD11c-CD18 (alpha(x)beta(2)) integrins, which promote their adhesion and activation, For monocyte activation, specific domains of the type I collagen telopeptides are necessary, Interactions between monocytes and collagen are most likely involved in the cascade of events that characterize the initial phases of inflammation.