Increased transcription of the tyrosine hydroxylase gene in individual locus coeruleus neurons following footshock stress
Increased transcription of the tyrosine hydroxylase gene in individual locus coeruleus neurons following footshock stress
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DOI：
10.1016/s0306-4522(00)00352-3
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发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Austin, MC
中科院分区：
文献类型：
--
作者：
Chang, MS;Sved, AF;Austin, MC
采用内含子特异性原位杂交组织化学技术检测足震引起的蓝斑神经元酪氨酸羟化酶转录活性的变化。在间断足震30分钟后3小时，发现蓝斑神经元中酪氨酸羟化酶初级转录物的细胞浓度显著增加。然而，足震诱导的酪氨酸羟化酶转录增加在蓝斑神经元中并不均匀表达。同样，给药α(2)-肾上腺素能受体拮抗剂咪唑嗪可显著增加酪氨酸羟化酶初级转录本的细胞浓度，这些转录本在蓝斑神经元中呈异质性分布。足震和咪唑嗪均显著提高蓝斑区酪氨酸羟化酶信使RNA的区域水平。暴露于足震15分钟后，检测蓝斑处酪氨酸羟化酶转录率和信使RNA水平变化的时间过程。在脚震15分钟结束时，酪氨酸羟化酶转录率显著增加，在6小时内保持升高，并在24小时内恢复到对照水平。在脚震15分钟后，蓝斑神经元的酪氨酸羟化酶信使RNA浓度直到6小时才增加，24小时时仍保持升高。这些研究结果表明，蓝斑神经元中酪氨酸羟化酶基因在脚震应激下的转录发生迅速，持续时间长，且分布不均匀。这些数据还表明，足震后酪氨酸羟化酶信使RNA的增加至少部分是由酪氨酸羟化酶基因转录的增加介导的。(c) 2000 ibro。Elsevier Science Ltd.出版。版权所有。
Footshock-evoked change in transcriptional activity of tyrosine hydroxylase in neurons of the locus coeruleus was examined using an intron-specific in situ hybridization histochemical technique. A significant increase in the cellular concentration of tyrosine hydroxylase primary transcripts was found in locus coeruleus neurons 3 h following 30 min of intermittent footshock. However, the footshock-induced increase in tyrosine hydroxylase transcription was not homogeneously expressed in locus coeruleus neurons. Similarly, administration of the alpha (2)-adrenergic receptor antagonist idazoxan produced a significant increase in the cellular concentration of tyrosine hydroxylase primary transcripts that was heterogeneously distributed among locus coeruleus neurons. Both footshock and idazoxan significantly increased the regional levels of tyrosine hydroxylase messenger RNA in the locus coeruleus. The time-course of changes in tyrosine hydroxylase transcription rate and messenger RNA levels in the locus coeruleus was examined after a 15 min exposure to footshock. A robust increase in tyrosine hydroxylase transcription rate was found at the end of 15 min of footshock, which remained elevated for 6 h and was back to the control levels by 24 h. In contrast, in response to a 15 min period of footshock tyrosine hydroxylase messenger RNA concentrations in the locus coeruleus did not increase until 6 h and remained elevated at 24 h.These findings demonstrate that transcription of the tyrosine hydroxylase gene in locus coeruleus neurons in response to footshock stress occurs rapidly, is sustained for many hours and is heterogeneously distributed. These data also suggest that the increase in tyrosine hydroxylase messenger RNA following footshock is mediated, at least in part, by an increase in tyrosine hydroxylase gene transcription. (C) 2000 IBRO. Published by Elsevier Science Ltd. All rights reserved.