ENV AND VPU PROTEINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ARE PRODUCED FROM MULTIPLE BICISTRONIC MESSENGER-RNAS
ENV AND VPU PROTEINS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ARE PRODUCED FROM MULTIPLE BICISTRONIC MESSENGER-RNAS
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DOI：
10.1128/jvi.64.11.5448-5456.1990
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发表时间：
1990-11-01
影响因子：
5.4
通讯作者：
PAVLAKIS, GN
中科院分区：
文献类型：
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作者：
SCHWARTZ, S;FELBER, BK;PAVLAKIS, GN
人类免疫缺陷病毒1型（HIV-1）mRNA在感染细胞中产生三种大小类别：全长、中等和小。在这里，我们报告说，中等大小的类病毒mRNA是异质性的，由至少12个差异剪接的物种。该组包含9个产生Env和Vpu的双顺反子mRNA和3个仅表达达特的第一外显子的mRNA。在后者的mRNA中，Env和Vpu的表达因上游达特开放阅读框的存在而被阻断。我们的结论是，内部启动的翻译是不是双顺反子的env mRNA的产生机制。HIV-1 mRNA的翻译与扫描机制一致，其中Env是通过从含有env作为第二或第三阅读框的mRNA进行泄漏扫描而产生的。Env和Vpu蛋白由相同的mRNA表达，并由Rev协调调节。这种安排可能反映了Vpu和Env协调表达的要求。
Three size classes of human immunodeficiency virus type 1 (HIV-1) mRNAs are produced in infected cells: full-length, intermediate, and small. Here we report that the intermediate-size class of viral mRNAs is heterogeneous, consisting of at least 12 differentially spliced species. This group contains nine bicistronic mRNAs producing Env and Vpu and three mRNAs expressing only the first exon of tat. In the latter mRNAs, Env and Vpu expression is blocked by the presence of the upstream tat open reading frame. We conclude that internal initiation of translation is not the mechanism for generation of the bicistronic env mRNAs. Translation of HIV-1 mRNAs is consistent with the scanning mechanism in which Env is produced by leaky scanning from mRNAs that contain env as the second or third reading frame. Env and Vpu proteins are expressed from the same mRNAs and are coordinately regulated by Rev. This arrangement may reflect a requirement for coordinate expression of Vpu and Env.