Expression of transforming growth factor beta (TGF beta) receptors and expression of TGF beta 1, TGF beta 2 and TGF beta 3 in human small cell lung cancer cell lines.
Expression of transforming growth factor beta (TGF beta) receptors and expression of TGF beta 1, TGF beta 2 and TGF beta 3 in human small cell lung cancer cell lines.
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DOI：
10.1038/bjc.1993.186
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发表时间：
1993-05
影响因子：
8.8
通讯作者：
Skovgaard Poulsen, H
中科院分区：
文献类型：
--
作者：
Damstrup, L;Rygaard, K;Spang-Thomsen, M;Skovgaard Poulsen, H
检测了一组21个小细胞肺癌细胞（SCLC）系中转化生长因子β受体（TGF β-r）的存在和TGF β mRNA的表达。通过放射受体分析，我们发现高亲和力受体在六个细胞系中表达。结合数据的Scatchard分析表明，细胞结合4.5至27.5fmolmg-1蛋白，KD范围为16至40 pM。通过将TGF β 1与TGF β-γ交联来证实TGF β 1与受体的结合。证明了三种类型的TGF β-r，I型和II型受体，M（r）= 65，000和90，000，β聚糖（III型），M（r）= 280，000。北方印迹法显示10株细胞表达TGF β 1 mRNA，2株表达TGF β 2 mRNA，7株表达TGF β 3 mRNA。我们的研究结果第一次提供了证据，证明一组广泛的SCLC细胞系中有很大一部分表达TGF β受体，并且也产生TGF β mRNA。
A panel of 21 small cell lung cancer cell (SCLC) lines were examined for the presence of Transforming growth factor beta receptors (TGF beta-r) and the expression of TGF beta mRNAs. By the radioreceptor assay we found high affinity receptors to be expressed in six cell lines. scatchard analysis of the binding data demonstrated that the cells bound between 4.5 and 27.5 fmol mg-1 protein with a KD ranging from 16 to 40 pM. TGF beta 1 binding to the receptors was confirmed by cross-linking TGF beta 1 to the TGF beta-r. Three classes of TGF beta-r were demonstrated, type I and type II receptors with M(r) = 65,000 and 90,000 and the betaglycan (type III) with M(r) = 280,000. Northern blotting showed expression of TGF beta 1 mRNA in ten, TGF beta 2 mRNA in two and TGF beta 3 mRNA in seven cell lines. Our results provide, for the first time, evidence that a large proportion of a broad panel of SCLC cell lines express TGF beta-receptors and also produce TGF beta mRNAs.