Microbiological Corrosion: Hydrogen Permeation and Sulfate-Reducing Bacteria
Microbiological Corrosion: Hydrogen Permeation and Sulfate-Reducing Bacteria
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DOI：
10.5006/1.3277633
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发表时间：
2002-05
期刊：
影响因子：
1.6
通讯作者：
M. Romero;Z. Duque;O. D. Rincón;O. Pérez;Ismenia Araujo;Beltrán Briceño
中科院分区：
文献类型：
--
作者：
M. Romero;Z. Duque;O. D. Rincón;O. Pérez;Ismenia Araujo;Beltrán Briceño
摘要本研究旨在探讨硫酸盐还原菌（SRB）在微生物腐蚀（MIC）中的作用机制--阴极去极化。脱硫菌ATCC 7757。使用接种或未接种10%D的脱气无菌培养基进行渗透试验。在108个细胞/mL下，采用连续稀释法评价细菌生长情况，采用扫描电子显微镜（SEM）分析Pd箔上形成的生物膜和产物的特征。结果表明，在极化和非极化试验中，24 h时细菌生长量均为4 × 1010 CFU/mL，而无阴极极化的氢渗透试验确定不存在还原硫化氢（H2S）分解产生的H+的条件。
Abstract This study was undertaken to evaluate cathodic depolarization as the action mechanism triggered by sulfate-reducing bacteria (SRB) in microbiologically induced corrosion (MIC), using an inert substrate such as a 1-mm thick Pd foil with and without cathodic polarization, a H0 permeation conventional Devanathan-type cell, and the bacteria Desulfovibrio desulfuricans subsp. desulfuricans ATCC 7757. The permeation tests were run using a deaerated sterile culture medium inoculated or not with 10% D. desulfuricans at 108 cell/mL. Serial dilution was used to evaluate the bacterial growth, and scanning electron microscopy (SEM) was used to analyze the characteristics of the biofilm and products formed on the Pd foil. Results indicated bacterial growth on the order of 4 × 1010 CFU/mL at 24 h in both polarization and nonpolarization tests, and hydrogen permeation tests without cathodic polarization determined that there were no conditions for the reduction of the H+ generated by hydrogen sulfide (H2S) diss...