Gene expression of human squamous cell carcinoma antigens 1 and 2 in human cell lines.
Gene expression of human squamous cell carcinoma antigens 1 and 2 in human cell lines.
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人类鳞状细胞癌抗原 1 和 2 在人类细胞系中的基因表达。
DOI：
10.3892/or.8.2.347
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发表时间：
2001
期刊：
影响因子：
4.2
通讯作者：
M. Ito
中科院分区：
文献类型：
--
作者：
K. Hamada;Y. Hanakawa;K. Hashimoto;M. Iwamoto;T. Kihana;S. Hirose;M. Nakamura;M. Ito
鳞状细胞癌抗原（SCCA）作为鳞状细胞癌的血清学标志物。SCCA基因组区域的分子克隆显示存在两个串联排列的基因，SCCA1和SCCA2。SCCA1是一种木瓜蛋白酶样半胱氨酸蛋白酶抑制剂，而SCCA2是一种凝乳胰蛋白酶样丝氨酸蛋白酶抑制剂。关于SCCA1和SCCA2基因的表达在人类细胞系中是如何调控的，我们知之甚少。本研究的目的是确定SCCA1基因还是SCCA2基因在人类细胞系中表达更强烈。经酶联免疫吸附法测定，鳞状细胞癌细胞向培养基中分泌的SCCA蛋白分别是正常人角质形成细胞和非鳞状细胞癌细胞的4倍和50倍。定量RT-PCR ELISA法显示，SCCA1 mRNA在鳞状细胞癌细胞系中的表达量分别是角质形成细胞和非鳞状细胞癌细胞系的2.8倍和42倍。SCCA1与SCCA2 mRNA的表达比例在鳞状细胞癌、角质细胞癌和非鳞状细胞癌细胞系中存在明显差异（鳞状细胞癌2.8；角质细胞癌24.1；非鳞状细胞癌11.0）。这些发现提示SCCA1主要在鳞状细胞癌、角化细胞和非鳞状细胞癌细胞系中表达，SCCA1与SCCA2的表达比例可能是检测鳞状细胞癌的一种新的标志物。
The squamous cell carcinoma antigen (SCCA) serves as a serological marker for squamous cell carcinomas. Molecular cloning of the SCCA genomic region has revealed the presence of two tandemly arrayed genes, SCCA1 and SCCA2. SCCA1 is a papain-like cysteine proteinase inhibitor, while SCCA2 is a chymotrypsin-like serine proteinase inhibitor. Little is known concerning how expression of the SCCA1 and SCCA2 genes is regulated in human cell lines. The purpose of this study was to determine whether the SCCA1 gene or SCCA2 gene is more strongly expressed in human cell lines. Squamous cell carcinoma cell lines secreted respectively 4 times and 50 times as much SCCA proteins into medium as normal human keratinocyte and non-squamous cell carcinoma cell lines, as measured by enzyme-linked immunosorbent assay. Quantitative RT-PCR ELISA digoxigenin-labeling assay demonstrated that SCCA1 mRNA expression in squamous cell carcinoma cell lines was respectively 2.8 times and 42 times that in keratinocyte and non-squamous cell carcinoma cell lines. The ratio of SCCA1 to SCCA2 mRNA expression differed distinctly among squamous cell carcinoma, keratinocyte and non-squamous cell carcinoma cell lines (2.8, squamous; 24.1, keratinocyte; 11.0, non-squamous). These findings suggest that SCCA1 is mainly expressed in squamous cell carcinoma, keratinocyte and non-squamous cell carcinoma cell lines and that the ratio of SCCA1 to SCCA2 expression might be a novel marker for the detection of squamous cell carcinoma.