Molecular genetic analysis of black poplar (Populus nigra L.) along Dutch rivers
Molecular genetic analysis of black poplar (Populus nigra L.) along Dutch rivers
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DOI：
10.1046/j.1365-294x.1998.00316.x
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发表时间：
1998-01-01
影响因子：
4.9
通讯作者：
Vosman, B
中科院分区：
文献类型：
--
作者：
Arens, P;Coops, H;Vosman, B
利用AFLP技术对荷兰莱茵河支流河岸残存的黑白杨（Populusnigra）的遗传结构进行了研究。共对143株杨树进行了AFLP分析，其中包括1株美洲黑杨和部分欧美黑杨，共产生319条多态性条带。AFLP分析表明，部分黑杨样本间存在明显差异。对于美洲黑杨树和所有已经被鉴定为杂交美洲黑杨的树也观察到这些偏离模式。这两个物种之间的杂交在形态上有时很难与物种本身区分开来。莱茵河河岸的两个重要的可能来源种群，由成熟的开花的黑杨树组成，似乎只由几个基因型组成。相比之下，年轻的黑白杨树单独生长或在可能的源种群下游的小团体似乎主要是generatively派生，因为没有成熟的树木被发现在他们中间的克隆。因此，无性繁殖似乎是一种非常局部的策略，而新地区的殖民似乎是通过生殖繁殖发生的。这些黑杨的遗传多样性是否足以维持河岸的生态化和集合种群的生存是一个有待进一步研究的问题。
The genetic structure of remaining black poplar (Populus nigra) trees on the banks of the Dutch Rhine branches was investigated using the AFLP technique. In total, 143 trees, including one P. deltoides and some P. x euramericana, were analysed using six AFLP primer combinations which generated 319 polymorphic bands. The AFLP patterns showed that some of the trees sampled as P. nigra were clearly different. These deviating patterns were also observed for the P. deltoides tree and all trees already identified as hybrid P. x euramericana. Hybrids between the two species are morphologically sometimes difficult to distinguish from the species itself. Two important possible source populations for recolonization of the riverbanks of the river Rhine, consisting of mature flowering P. nigra trees, appeared to consist of only a few genotypes each. In contrast, young black poplar trees growing alone or in small groups downstream of the possible source populations appeared to be predominantly generatively derived because no clones of mature trees were found among them. Therefore vegetative propagation seems a very local strategy whereas colonization of new areas appears to occur through generative propagation. Whether the genetic diversity within these black poplars is sufficient for recolonization of river banks and survival of the metapopulation is a question for further research.