Inactivation of influenza A virus H1N1 by disinfection process
Inactivation of influenza A virus H1N1 by disinfection process
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DOI：
10.1016/j.ajic.2010.03.003
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发表时间：
2010-06-01
影响因子：
4.9
通讯作者：
Kim, In Seop
中科院分区：
文献类型：
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作者：
Jeong, Eun Kyo;Bae, Jung Eun;Kim, In Seop
背景资料：由于任何患者、医护人员或访客都有可能将流感传播给医院内的易感人群，因此医院获得性流感一直是临床关注的问题。对医疗设备、手术器械和医院环境进行消毒和清洁是防止流感病毒从医院传播给个人的重要措施。本研究旨在评价医院易于操作的消毒工艺对甲型H1N1流感病毒（H1N1）的灭活效果。方法：研究了0.1 mol/L NaOH、70%乙醇、70%正丙醇、0.3%磷酸三丁酯和1.0%Triton X-100组成的溶剂/去污剂（S/D）、加热、和环氧乙烷（EO）处理对甲型H1N1流感病毒的灭活作用。通过消毒剂对病毒溶液的处理，动力学地确定了H1N1的灭活。此外，表面测试方法，其中涉及干燥的表面上的病毒的量，然后应用灭活方法为1分钟的接触时间，被用来确定virucidal activity.Results：H1N1完全灭活到不可检测的水平在1分钟的70%乙醇，70%的1-丙醇，和溶剂/洗涤剂处理的表面测试，以及在悬浮液测试。H1N1在悬浮液试验中在0.1 mol/L NaOH处理1分钟内完全灭活，在表面试验中也有效灭活，对数减少因子为3.7。在悬浮液试验中，H1N1分别在70、80和90摄氏度的热处理5分钟、2.5分钟和1分钟内被灭活至检测不到的水平。此外，H1N1完全灭活EO处理在表面tests.Conclusion：常见的消毒剂，热，和EO在这项研究中测试的灭活H1N1是有效的。这些结果将有助于实施有效的消毒措施以预防医院获得性感染。
Background: Because any patient, health care worker, or visitor is capable of transmitting influenza to susceptible persons within hospitals, hospital-acquired influenza has been a clinical concern. Disinfection and cleaning of medical equipment, surgical instruments, and hospital environment are important measures to prevent transmission of influenza virus from hospitals to individuals. This study was conducted to evaluate the efficacy of disinfection processes, which can be easily operated at hospitals, in inactivating influenza A virus H1N1 (H1N1).Methods: The effects of 0.1 mol/L NaOH, 70% ethanol, 70% 1-propanol, solvent/detergent (S/D) using 0.3% tri (n-butyl)-phosphate and 1.0% Triton X-100, heat, and ethylene oxide (EO) treatments in inactivating H1N1were determined. Inactivation of H1N1 was kinetically determined by the treatment of disinfectants to virus solution. Also, a surface test method, which involved drying an amount of virus on a surface and then applying the inactivation methods for 1 minute of contact time, was used to determine the virucidal activity.Results: H1N1 was completely inactivated to undetectable levels in 1 minute of 70% ethanol, 70% 1-propanol, and solvent/detergent treatments in the surface tests as well as in the suspension tests. H1N1 was completely inactivated in 1 minute of 0.1 mol/L NaOH treatment in the suspension tests and also effectively inactivated in the surface tests with the log reduction factor of 3.7. H1N1 was inactivated to undetectable levels within 5 minutes, 2.5 minutes, and 1 minute of heat treatment at 70, 80, and 90 degrees C, respectively in the suspension tests. Also, H1N1 was completely inactivated by EO treatment in the surface tests.Conclusion: Common disinfectants, heat, and EO tested in this study were effective at inactivating H1N1. These results would be helpful in implementing effective disinfecting measures to prevent hospital-acquired infections.