Characterization of the γ-aminobutyric acid shunt pathway and oxidative damage in Arabidopsis thaliana pop 2 mutants under various abiotic stresses
Characterization of the γ-aminobutyric acid shunt pathway and oxidative damage in Arabidopsis thaliana pop 2 mutants under various abiotic stresses
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DOI：
10.1007/s10535-015-0563-5
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发表时间：
2016-01-01
影响因子：
1.5
通讯作者：
Al-Share, A. T.
中科院分区：
文献类型：
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作者：
Al-Quraan, N. A.;Al-Share, A. T.
本研究以拟南芥（Arabidopsis thaliana）pop 2突变体为材料，在高温等非生物胁迫条件下，对3个GABA-TA突变体的种子发芽率、胁迫诱导的氧化损伤、GABA含量和代谢进行了研究（42 A ° C）、低温（4A ° C）、盐度（NaCl）和渗透胁迫（甘露醇）。所有的突变株系在所有的胁迫处理下都表现出降低的发芽率，其中pop 2 -1和pop 2 -3突变株系的发芽率显著降低。GABA和MDA含量在所有突变体和野生型（WT）幼苗响应于所有处理显着增加。在相同的处理条件下，突变体的GABA和MDA含量均低于野生型。GABA在30分钟后已经增加，并且在42 A ° C下2小时后显著增加，特别是在pop 2 -3和WT幼苗中。响应于冷处理，在所有pop 2突变体和WT幼苗中，GABA含量与对照相比增加高达4倍。响应于NaCl处理，GABA在WT和所有pop 2突变体中略有积累。相反，在所有甘露醇处理下，在pop 2、pop 2 -1和pop 2 -3突变体和WT中GABA含量显著增加。
In the present study, three Arabidopsis thaliana pop2 mutant lines with different T-DNA insertions in a gene coding gamma-aminobutyric acid transaminase (GABA-TA) were screened for seed germination percentage, stress-induced oxidative damage, and GABA content and metabolism under various abiotic stresses including high temperature (42 A degrees C), low temperature (4 A degrees C), salinity (NaCl), and osmotic stress (mannitol). All mutant lines showed a decreased germination under all the stress treatments with a significant reduction in the pop2-1 and pop2-3 mutant lines. Content of GABA and MDA increased significantly in all pop2 mutants and wild type (WT) seedlings in response to all the treatments. However, content of GABA and MDA was lower in all pop2 mutants comparing to the WT under the same treatments. GABA increased already after 30 min and increased significantly after 2 h at 42 A degrees C especially in the pop2-3 and WT seedlings. In response to the cold treatment, GABA content increased up to 4-fold compared to the control in all pop2 mutants and WT seedlings. In response to the NaCl treatment, GABA accumulated slightly in the WT and all pop2 mutants. On the contrary, GABA content increased significantly in the pop2, pop2-1, and pop2-3 mutants and WT under all mannitol treatments.