Enhancer-promoter communication at the Drosophila engrailed locus
Enhancer-promoter communication at the Drosophila engrailed locus
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DOI：
10.1242/dev.036426
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发表时间：
2009-09-15
期刊：
影响因子：
4.6
通讯作者：
Kassis, Judith A.
中科院分区：
文献类型：
--
作者：
Kwon, Deborah;Mucci, Diane;Kassis, Judith A.
增强子通常位于距离它们调节的启动子数十个碱基的位置，有时更靠近相邻基因的启动子。他们怎么知道激活哪个基因？我们已经使用归巢P[en]构建体来研究增强子-启动子通信在enrailed位点。在这里，我们表明，enrailed增强子可以在很大的距离，甚至跳过其他转录单位，选择enrailed启动子的邻近基因。这种特异性至少以三种方式实现。首先，早期作用的锯齿状增强子表现出启动子特异性。第二，近端启动子-栓系元件对于胚盘增强子的作用是必需的。我们的数据表明，有两个部分冗余的启动子拴系元素。第三，长距离的作用需要一个组合的啮合启动子和序列内或紧密连接到启动子近端Polycomb组响应元件的啮合增强子。这些数据表明，多种机制，确保适当的增强子-启动子通信在果蝇enrailed基因座。
Enhancers are often located many tens of kilobases away from the promoter they regulate, sometimes residing closer to the promoter of a neighboring gene. How do they know which gene to activate? We have used homing P[en] constructs to study the enhancer-promoter communication at the engrailed locus. Here we show that engrailed enhancers can act over large distances, even skipping over other transcription units, choosing the engrailed promoter over those of neighboring genes. This specificity is achieved in at least three ways. First, early acting engrailed stripe enhancers exhibit promoter specificity. Second, a proximal promoter-tethering element is required for the action of the imaginal disc enhancer(s). Our data suggest that there are two partially redundant promoter-tethering elements. Third, the long-distance action of engrailed enhancers requires a combination of the engrailed promoter and sequences within or closely linked to the promoter proximal Polycomb-group response elements. These data show that multiple mechanisms ensure proper enhancer-promoter communication at the Drosophila engrailed locus.