Effects of tumor promoters and extracellular calcium on the growth of normal, transformed and temperature sensitive rat liver epithelial cells.
Effects of tumor promoters and extracellular calcium on the growth of normal, transformed and temperature sensitive rat liver epithelial cells.
复制标题
肿瘤促进剂和细胞外钙对正常、转化和温度敏感的大鼠肝上皮细胞生长的影响。
DOI：
10.1016/0304-3835(80)90059-2
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发表时间：
1980
期刊：
影响因子：
9.7
通讯作者：
Weinstein,IB
中科院分区：
文献类型：
--
作者：
Fisher,PB;Weinstein,IB
我们比较了细胞外Ca 2+浓度和佛波酯肿瘤促进剂对正常大鼠肝上皮细胞生长的影响，这些细胞的化学转化衍生物和后者细胞的突变体是温度敏感的表达转化。正常的K16或K22细胞在1.25 mM Ca 2+中比在0.01 mM Ca 2+中生长得好得多，而它们的转化对应物W8在1.25和0.01 mM Ca 2+中生长得同样好。温度敏感突变体TS 223表现出中间表型。当在36°C或39.5°C下生长时，这些细胞比K16或K22细胞更少地依赖于Ca 2 +;但它们比W8细胞更依赖于Ca 2+。佛波酯肿瘤促进剂TPA（12-O-十四烷酰基-佛波醇-13-乙酸酯）、PDD（佛波醇-12，13-二癸酸酯）和PDB（佛波醇-12，13-二苯甲酸酯）增强K16或K22细胞在0.01mM Ca 2+中的生长。另一方面，在36°C和39.5°C下，在低（0.01 mM）和正常（1.25 mM）Ca 2+浓度下，相同的试剂显著抑制W8和TS 223细胞的生长。两种结构相关的二萜类化合物（对小鼠皮肤缺乏肿瘤促进活性）佛波醇和4αPDD在低或正常Ca 2+浓度下均未改变任何这些细胞系的生长特性。因此，我们的研究结果表明，正常和转化的大鼠肝上皮细胞的细胞外Ca 2+和肿瘤促进剂的生长控制之间的反应的差异。
We have compared the effect of extracellular Ca2+concentration and of phorbol ester tumor promoters on the growth of normal rat liver epithelial cells, a chemically transformed derivative of these cells and a mutant of the latter cells that is temperature sensitive in the expression of transformation. The normal K16 or K22 cells grew considerably better in 1.25 mM Ca2+than in 0.01 mM Ca2+, whereas their transformed counterpart, W8, grew equally well in 1.25 and 0.01 mM Ca2+. The temperature-sensitive mutant TS 223 exhibited an intermediate phenotype. When grown at either 36°C or 39.5°C, these cells were less Ca2+dependent than K16 or K22 cells; but they were more Ca2+dependent than W8 cells. The phorbol ester tumor promoters TPA (12-O-tetradecanoyl-phorbol-13-acetate), PDD (phorbol-12, 13-didecanoate) and PDB (phorbol-12,13-dibenzoate) enhanced the growth of K16 or K22 cells in 0.01 mM Ca2+. On the other hand, the same agents markedly inhibited the growth of W8 and TS 223 cells, at 36°C and 39.5°C, in both low (0.01 mM) and normal (1.25 mM) Ca2+concentrations. Two structurally related diterpenes which lack tumor promoting activity on mouse skin, phorbol and 4αPDD, did not alter the growth properties of any of these cell lines, at either low or normal Ca2+concentrations. Our results indicate, therefore, differences in the responses between normal and transformed rat liver epithelial cells to growth control by extracellular Ca2+and tumor promoters.