Enhanced Laccase Production in White-Rot Fungus Rigidoporus lignosus by the Addition of Selected Phenolic and Aromatic Compounds
Enhanced Laccase Production in White-Rot Fungus Rigidoporus lignosus by the Addition of Selected Phenolic and Aromatic Compounds
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DOI：
10.1007/s12010-010-9049-2
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发表时间：
2011-02-01
影响因子：
3
通讯作者：
Cambria, Antonio
中科院分区：
文献类型：
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作者：
Cambria, Maria Teresa;Ragusa, Santa;Cambria, Antonio
白腐菌产生大量的细胞外漆酶，这是一种多铜蓝氧化酶，能够氧化各种有机底物。在补充了各种芳香和酚类化合物的液体培养基中，漆酶的产量可以大大提高。添加诱导剂后，真菌培养第21天或第24天酶活性达到最大值。在诱导物存在的情况下，培养制剂的细胞外液酶谱图显示，在最大活性日，Lac1和Lac2两条具有不同强度的酶活性条带。Lac2波段强度随诱导剂的不同而变化。在橄榄厂废水中添加酚类化合物含量高的培养基也能诱导漆酶。
The white rot fungus Rigidoporus lignosus produces substantial amounts of extracellular laccase, a multicopper blue oxidase which is capable of oxidizing a wide range of organic substrates. Laccase production can be greatly enhanced in liquid cultures supplemented with various aromatic and phenolic compounds. The maximum enzyme activity was reached at the 21st or 24th day of fungal cultivation after the addition of inducers. The zymograms of extracellular fluid of culture preparation in the presence of inducers, at maximum activity day, revealed two bands with enzymatic activity, called Lac1 and Lac2, having different intensities. Lac2 band shows the higher intensity which changed with the different inducers. Laccase induction can be also obtained by adding to the culture medium olive mill wastewaters, which shows a high content of phenolic compounds.