Capsid protein diversity among Norwalk-like viruses
Capsid protein diversity among Norwalk-like viruses
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DOI：
10.1023/a:1008140611929
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发表时间：
2000-05-01
期刊：
影响因子：
1.6
通讯作者：
Brown, DWG
中科院分区：
文献类型：
--
作者：
Green, J;Vinje, J;Brown, DWG
我们已经确定了完整的衣壳基因序列的20诺沃克样病毒（NLVs）收集主要从1989年和1996年之间在英国爆发。这些菌株包括9个基因组I和11个基因组II菌株。这些和15个已发表的序列的系统发育分析表明，在基因组I，包括三个先前描述的7个基因组亚组。在基因组II中，8个亚组是明显的，其中4个是新的。不同基因组菌株之间的氨基酸同源性为37%至44%，而同一基因组内菌株的氨基酸同源性为61%至100%。衣壳的N-末端和中央可变区的单独的系统发育分析显示出良好的相关性。菌株之间的序列差异是最大的中央可变区，氨基酸序列的同一性低至28%的基因组内。这15个基因组亚组为进一步研究杯状病毒分支内的遗传和抗原关系提供了框架。
We have determined the complete capsid gene sequence of 20 Norwalk-like viruses (NLVs) collected predominantly from outbreaks in the UK between 1989 and 1996. These comprised nine genogroup I and eleven genogroup II strains. Phylogenetic analysis of these and 15 published sequences suggest seven genomic sub-groups within genogroup I, including three previously described. In genogroup II, eight sub-groups were apparent, of which four were novel. Amino acid identities between strains of distinct genogroups ranged from 37 to 44% while varying between 61 and 100% for strains within a genogroup. Separate phylogenetic analyses of the N-terminus and central variable region of the capsid showed good correlation. Sequence divergence between strains was greatest within the central variable region, with amino acid sequence identities as low as 28% within a genogroup. These 15 genomic sub-groups provide a framework for further investigations of genetic and antigenic relationships within this calicivirus clade.