Isolation and insecticidal activity of sesquiterpenes alkaloids from Tripterygium wilfordii Hook f.
Isolation and insecticidal activity of sesquiterpenes alkaloids from Tripterygium wilfordii Hook f.
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DOI：
10.1016/j.indcrop.2013.11.029
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发表时间：
2014
影响因子：
5.9
通讯作者：
Zhiqing Ma;Yangjun Li;Lipeng Wu;Xing Zhang
中科院分区：
文献类型：
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作者：
Zhiqing Ma;Yangjun Li;Lipeng Wu;Xing Zhang
分离植物次生代谢产生的化合物可导致新的杀虫药物的发现。鉴于这种可能性，我们从重要的中药材雷公藤根皮的乙醇提取物中分离出7种倍半萜生物碱。鉴定为雷公藤甲素、1-去乙酰雷公藤碱、雷公藤碱、1-去乙酰雷公藤碱、雷公藤碱、雷公藤碱甲。为了确定它们的生物活性，对不同的医学和农业重要害虫进行了生物测定。雷公藤甲素、1-去乙酰雷公藤甲素和雷公藤甲素对粘虫3龄幼虫均有较强的拒食作用(DC50分别为17.68、33.52和4.10nμg/ml)和小菜蛾。(鳞翅目：小菜蛾科)(DC50分别为54.62、55.19和36.65μg/ml)。分离物(EC_(50)分别为630.30、212.17和100.89μg/ml)和P.小菜蛾的EC_(50)分别为177.51、336.59和97.77g/μ。对淡色库蚊3龄幼虫和家蝇成虫的LC_(50)分别为25.70、25.40、22.58和14.57nμg/ml。(双翅目：蝇科)(LC_(50)分别为87.29、70.19、47.80和21.00μg/ml)。总体而言，雷公藤碱是分离得到的最具活性的倍半萜生物碱。柳树皮。这也是首次报道1-脱乙酰雷公藤碱和1-脱乙酰雷公藤碱的杀虫活性。它的潜在用途。讨论了植物源杀虫剂中的雷公藤二衍生物。
The isolation of compounds produced by secondary metabolism of plants can lead to the discovery of new insecticidal agents. In light of this possibility, we isolated seven sesquiterpenes alkaloids from an ethanolic extract ofTripterygium wilfordii(Celastraceae) root bark, an important Chinese medicinal species. These were identified as wilforjine, 1-desacetylwilforgine, wilforgine, 1-desacetylwilforine, wilforine, wilfortrine and peritassines A. To characterize their biological activities, bioassays were conducted using different insect pest species of medical and agricultural importance. Wilforgine, 1-desacetylwilforine, and wilforine showed strong antifeedant effects to 3rd instar larvae of bothMythimna separataWalker (Lepidoptera: Noctuidae) (DC50= 17.68, 33.52 and 4.10 μg/ml, respectively) andPlutella xylostellaL. (Lepidoptera: Plutellidae) (DC50= 54.62, 55.19 and 36.65 μg/ml, respectively) as well as knockdown activity in 3rd instar larvae of bothM. separata(EC50= 630.30, 212.17 and 100.89 μg/ml, respectively) andP. xylostella(EC50= 177.51, 336.59 and 97.77 μg/ml, respectively). Moreover, 1-desacetylwilforgine, wilforgine, 1-desacetylwilforine, and wilforine showed insecticidal activity to 3rd instar larvae ofCulex pipiens pallensCoquillett (Diptera: Culicidae) (LC50= 25.70, 25.40, 22.58 and 14.57 μg/ml, respectively) and adults ofMusca domesticaL. (Diptera: Muscidae) (LC50= 87.29, 70.19, 47.80 and 21.00 μg/ml, respectively). In general, wilforine was the most active sesquiterpene alkaloid isolated fromT. wilfordiiroot bark. This is also the first report of insecticidal activity of 1-desacetylwilforgine and 1-desacetylwilforine. The potential use ofT. wilfordiiderivatives in botanical insecticides is discussed.