House Dust Endotoxin and Peripheral Leukocyte Counts: Results from Two Large Epidemiologic Studies.
House Dust Endotoxin and Peripheral Leukocyte Counts: Results from Two Large Epidemiologic Studies.
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DOI：
10.1289/ehp661
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发表时间：
2017-05-31
影响因子：
10.4
通讯作者：
Zeldin DC
中科院分区：
文献类型：
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作者：
Fessler MB;Carnes MU;Salo PM;Wilkerson J;Cohn RD;King D;Hoppin JA;Sandler DP;Travlos G;London SJ;Thorne PS;Zeldin DC
外周血白细胞计数是炎症的生物标志物，与人类全因死亡率相关。尽管急性白细胞增多的原因已得到充分描述，但白细胞数量的慢性环境决定因素尚不清楚。我们研究了人类受试者的室内灰尘内毒素浓度与外周白细胞计数之间的关系。 2005-2006 年国家健康和营养检查调查 (NHANES) () 和农业肺健康研究 (ALHS;) 中通过线性回归评估了内毒素 - 白细胞关系。在 ALHS 中，我们使用带有相互作用项的回归模型测试了内毒素-白细胞关系中基因 [Toll 样受体 4 (TLR4)，编码内毒素受体]与环境的相互作用。内毒素浓度与白细胞总数之间存在统计学上显着的正相关关系[内毒素每变化 10 倍，估计变化，(95% CI: 0.070, )； ）在 NHANES 中。单核细胞、淋巴细胞和中性粒细胞也存在类似的正相关关系。分层分析揭示了哮喘和慢性阻塞性肺疾病可能改变效应。我们在 ALHS 中观察到类似的关联。对于总白细胞，ALHS 中存在暗示性证据，表明 rs4986790 和 rs4986791 定义的 TLR4 单倍型的次要等位基因携带者状态存在基因与环境相互作用（相互作用 ）。据我们所知，这是全国调查中第一份关于室内灰尘内毒素与白细胞计数之间关联的报告。这一发现在农业人口中得到了重复。外周血白细胞计数可能会受到不同环境中居住内毒素暴露的影响。 https://doi.org/10.1289/EHP661
The peripheral leukocyte count is a biomarker of inflammation and is associated with human all-cause mortality. Although causes of acute leukocytosis are well-described, chronic environmental determinants of leukocyte number are less well understood. We investigated the relationship between house dust endotoxin concentration and peripheral leukocyte counts in human subjects. The endotoxin–leukocyte relationship was evaluated by linear regression in the National Health and Nutrition Examination Survey (NHANES) 2005–2006 () and the Agricultural Lung Health Study (ALHS; ). In the ALHS, we tested for a gene [Toll-like Receptor 4 (TLR4), encoding the endotoxin receptor]-by-environment interaction in the endotoxin–leukocyte relationship using regression models with an interaction term. There is a statistically significant, positive association between endotoxin concentration and total leukocyte number [estimated change, (95% CI: 0.070, ) per 10-fold change in endotoxin; ) in the NHANES. Similar positive associations were found for monocytes, lymphocytes, and neutrophils. Stratified analyses revealed possible effect modification by asthma and chronic obstructive pulmonary disease. We observed similar associations in the ALHS. For total leukocytes, there was suggestive evidence in the ALHS of a gene-by-environment interaction for minor allele carrier status at the TLR4 haplotype defined by rs4986790 and rs4986791 (interaction ). This is, to our knowledge, the first report of an association between house dust endotoxin and leukocyte count in a national survey. The finding was replicated in a farming population. Peripheral leukocyte count may be influenced by residential endotoxin exposure in diverse settings. https://doi.org/10.1289/EHP661