TBX3 Directs Cell-Fate Decision toward Mesendoderm.
TBX3 Directs Cell-Fate Decision toward Mesendoderm.
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DOI：
10.1016/j.stemcr.2013.08.002
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发表时间：
2013
影响因子：
5.9
通讯作者：
Kleger, Alexander
中科院分区：
文献类型：
--
作者：
Weidgang, Clair E.;Russell, Ronan;Tata, Purushothama R.;Kuehl, Susanne J.;Illing, Anett;Mueller, Martin;Lin, Qiong;Brunner, Cornelia;Boeckers, Tobias M.;Bauer, Kerstin;Kartikasari, Apriliana E. R.;Guo, Yanchun;Radenz, Melanie;Bernemann, Christof;Weiss, Matthias;Seufferlein, Thomas;Zenke, Martin;Iacovino, Michelina;Kyba, Michael;Schoeler, Hans R.;Kuehl, Michael;Liebau, Stefan;Kleger, Alexander
细胞命运决定和多能性取决于关键转录调节因子的网络。最近的报告证明了多能性相关因子在早期谱系定型过程中的附加功能。 T-box 转录因子 TBX3 与调节胚胎干细胞自我更新和心脏发生有关。在这里，我们展示了 TBX3 在体外分化胚胎干细胞 (ESC) 以及小鼠和非洲爪蟾胚胎体内发育的中内胚层谱系规范过程中动态表达。 ESC 中的强制 TBX3 表达通过直接激活关键谱系规范因子并通过增强旁分泌 Nodal/Smad2 信号传导间接促进中内胚层规范。非洲爪蟾中的 TBX3 功能丧失分析强调了其对中内胚层谱系定型的要求。此外，我们发现非洲爪蟾原肠胚形成过程中 TBX3 和 Tbx2 之间存在功能冗余。综上所述，我们进一步定义了 TBX3 作用的各个方面，并将 TBX3 映射为原肠胚形成期间中内胚层转录程序的上游调节因子。 T-box转录因子TBX3参与早期胚胎事件 TBX3占据中内胚层形成的关键转录因子启动子 TBX3促进mESCs的中内胚层命运
Cell-fate decisions and pluripotency are dependent on networks of key transcriptional regulators. Recent reports demonstrated additional functions of pluripotency-associated factors during early lineage commitment. The T-box transcription factor TBX3 has been implicated in regulating embryonic stem cell self-renewal and cardiogenesis. Here, we show that TBX3 is dynamically expressed during specification of the mesendoderm lineages in differentiating embryonic stem cells (ESCs) in vitro and in developing mouse and Xenopus embryos in vivo. Forced TBX3 expression in ESCs promotes mesendoderm specification by directly activating key lineage specification factors and indirectly by enhancing paracrine Nodal/Smad2 signaling. TBX3 loss-of-function analyses in the Xenopus underline its requirement for mesendoderm lineage commitment. Moreover, we uncovered a functional redundancy between TBX3 and Tbx2 during Xenopus gastrulation. Taken together, we define further facets of TBX3 actions and map TBX3 as an upstream regulator of the mesendoderm transcriptional program during gastrulation. T-box transcription factor TBX3 is involved in early embryonic events Key transcription factor promoters of mesendoderm formation are occupied by TBX3 TBX3 promotes mesendodermal fate of mESCs
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影响因子：
14.9
作者：
Finger JH;Smith CM;Hayamizu TF;McCright IJ;Eppig JT;Kadin JA;Richardson JE;Ringwald M
通讯作者：
Ringwald M
影响因子：
10.5
作者：
Hoogaars, Willem M. H.;Engel, Angela;Christoffels, Vincent M.
通讯作者：
Christoffels, Vincent M.
影响因子：
64.8
作者：
Brennan, J;Lu, CC;Robertson, EJ
通讯作者：
Robertson, EJ
影响因子：
4.8
作者：
Kleger, Alexander;Busch, Tobias;Seufferlein, Thomas
通讯作者：
Seufferlein, Thomas
影响因子：
37.8
作者：
Kleger, Alexander;Seufferlein, Thomas;Liebau, Stefan
通讯作者：
Liebau, Stefan