High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa)
High-throughput multiplex microsatellite marker assay for detection and quantification of adulteration in Basmati rice (Oryza sativa)
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DOI：
10.1002/elps.200600646
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发表时间：
2007-07-01
期刊：
影响因子：
2.9
通讯作者：
Nagaraju, Javaregowda
中科院分区：
文献类型：
--
作者：
Archak, Sunil;Lakshminarayanareddy, V.;Nagaraju, Javaregowda
Basmati大米是一种非常特殊的香米，以其超长的谷粒和令人愉快的独特香味而闻名世界。传统的Basmati水稻品种仅限于印度次大陆的印度-恒河地区，据报道，在出口市场上经常掺杂杂交Basmati品种和长粒非Basmati品种。目前，还没有商业规模的技术来可靠地检测掺假。我们在这里报告了一个基于CE的多重微卫星标记检测以及定量掺假Basmati大米样品。单管检测多路复用八个微卫星位点，以产生品种特异性等位基因谱，可以检测1%以上的掺假。该方案还纳入了定量竞争性PCR为基础的分析定量掺假。已证明定量准确度为+/-1.5%。用于开发和验证的方法的实验进行了说明。
Basmati rice is a very special type of aromatic rice known world-wide for its extra long grains and pleasant and distinct aroma. Traditional Basmati rice cultivars, confined to Indo-Gangetic regions of the Indian subcontinent, are often reported to be adulterated with crossbred Basmati varieties and long-grain non-Basmati varieties in the export market. At present, there is no commercial scale technology to reliably detect adulteration. We report here a CE-based multiplex microsatellite marker assay for detection as well as quantification of adulteration in Basmati rice samples. The single-tube assay multiplexes eight microsatellite loci to generate variety-specific allele profiles that can detect adulteration from 1% upwards. The protocol also incorporates a quantitative-competitive PCR-based analysis for quantification of adulteration. Accuracy of quantification has been shown to be +/- 1.5%. The experiments used to develop and validate the methodology are described.