Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr gene.
Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr gene.
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DOI：
10.1016/s0378-1119(02)00380-3
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发表时间：
2002-02
期刊：
影响因子：
3.5
通讯作者：
B. Blagoev;M. Nielsen;M. Angrist;A. Chakravarti;A. Pandey
中科院分区：
文献类型：
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作者：
B. Blagoev;M. Nielsen;M. Angrist;A. Chakravarti;A. Pandey
胸腺基质衍生淋巴生成素受体(TSLPR)是一种新的受体亚基，与白细胞介素2受体共同的伽马链序列相关。TSLPR与IL-7受体α链形成异二聚体复合体，形成胸腺基质来源淋巴生成素的受体，胸腺基质来源淋巴生成素是一种参与B细胞和T细胞功能的细胞因子。我们克隆了大鼠的TSLP受体，发现细胞因子受体胞外区的WSXWX基序在小鼠、大鼠和人的TSLP受体中以W(T/S)XV(T/A)基序保守。与小鼠一样，TSLP受体在大鼠中广泛表达，表明TSLPR可能在造血系统外的信号传递中发挥作用。Zoobot分析表明，TSLPR在所有被研究的脊椎动物中都有表达。TSLPR在酿酒酵母、果蝇和线虫基因组中的缺失与迄今已鉴定的其他几种细胞因子受体的表达相似。我们还对小鼠Tslpr基因的基因组结构进行了表征，结果表明，除了初级序列同源性外，它还与小鼠IL-2受体共同的伽马链(IL2RG)具有共同的编码外显子的基因组组织。使用选择性剪接受体位点导致小鼠TSLPR的两个选择性剪接转录本变体，这两个变体都是功能性受体。最后，通过连锁分析，我们将小鼠Tslpr基因定位于小鼠5号染色体ECC2和PXN基因之间。
Thymic stromal derived lymphopoietin receptor (TSLPR) is a novel receptor subunit that is related in sequence to the interleukin (IL)-2 receptor common gamma chain. TSLPR forms a heterodimeric complex with the IL-7 receptor alpha chain to form the receptor for thymic stromal derived lymphopoietin, a cytokine involved in B- and T-cell function. We have cloned the TSLP receptor from rat and find that the WSXWX motif commonly found in extracellular domains of cytokine receptors is conserved as a W(T/S)XV(T/A) motif among TSLP receptors from mouse, rat and human. As in the mouse, TSLP receptor is widely expressed in rats suggesting that TSLPR may have roles in signaling outside the hematopoietic system. A zooblot analysis revealed that TSLPR is expressed in all vertebrate species examined. The absence of TSLPR in Saccharomycescerevisiae, Drosophilamelanogaster and Caenorhabditiselegans genomes is similar to the expression of several other cytokine receptors that have been characterized thus far. We have also characterized the genomic structure of the murine Tslpr gene which shows that in addition to primary sequence homology, it shares a common genomic organization of coding exons with the murine IL-2 receptor common gamma chain (Il2rg). Use of an alternative splice acceptor site leads to two alternatively spliced transcript variants of murine TSLPR, both of which are functional receptors. Finally, using linkage analysis, we mapped the murine Tslpr gene to mouse chromosome 5 between the Ecm2 and Pxn genes.