Effect of Pore Architecture on Oxygen Diffusion in 3D Scaffolds for Tissue Engineering
Effect of Pore Architecture on Oxygen Diffusion in 3D Scaffolds for Tissue Engineering
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DOI：
10.1115/1.4002429
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发表时间：
2010-10-01
影响因子：
1.7
通讯作者：
Cho, Dong-Woo
中科院分区：
文献类型：
--
作者：
Ahn, Geunseon;Park, Jeong Hun;Cho, Dong-Woo
本研究的目的是最大化氧在三维支架内的扩散，以提高细胞活力和增殖。为了评估孔隙结构对氧气扩散的影响，我们设计了一种直径均匀的规则通道形状，称为圆柱形，以及一种具有通道直径梯度的新通道形状，称为锥形。数值分析预测，在整个支架中，锥形通道中的氧浓度高于圆柱形通道中的氧浓度。为了证实这些数值结果，我们检测了2D构建体和3D支架中的细胞增殖和活力。细胞培养实验表明，具有锥形通道的构建物和支架具有较好的细胞增殖能力和生存能力。(DOI: 10.1115/1.4002429)
The aim of this study was to maximize oxygen diffusion within a three-dimensional scaffold in order to improve cell viability and proliferation. To evaluate the effect of pore architecture on oxygen diffusion, we designed a regular channel shape with uniform diameter, referred to as cylinder shaped, and a new channel shape with a channel diameter gradient, referred to as cone shaped. A numerical analysis predicted higher oxygen concentration in the cone-shaped channels than in the cylinder-shaped channels, throughout the scaffold. To confirm these numerical results, we examined cell proliferation and viability in 2D constructs and 3D scaffolds. Cell culture experiments revealed that cell proliferation and viability were superior in the constructs and scaffolds with cone-shaped channels. [DOI: 10.1115/1.4002429]