Primary induction of metallothionein by dexamethasone in culture rat hepatocytes.
Primary induction of metallothionein by dexamethasone in culture rat hepatocytes.
复制标题
地塞米松在培养的大鼠肝细胞中初次诱导金属硫蛋白。
DOI：
10.1016/0006-291x(80)90808-6
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
D. Weinstein
中科院分区：
文献类型：
--
作者：
M. Karin;H. Herschman;D. Weinstein
地塞米松或锌离子可提高肝细胞培养中金属结合蛋白金属硫蛋白的合成速度。放线菌酮不能阻断地塞米松诱导金属硫蛋白合成的能力。相反，地塞米松刺激的锌摄取增加被放线菌亚胺抑制。和锌离子一样，地塞米松也是金属硫蛋白的“初级诱导剂”。原代培养的大鼠肝细胞糖皮质激素对金属硫蛋白的诱导不是通过增加锌摄取来实现的。
Dexamethasone or Zn++increase the rate of synthesis of the metal-binding protein metallothionein in hepatocyte cultures. Dexamethasone induction of the capacity to synthesize metallothionein is not blocked by cycloheximide. In contrast, the dexamethasone stimulated increase in Zn++uptake is inhibited by cycloheximide. Like Zn++, dexamethasone is a “primary inducer” of metallothionein. The glucocorticoid induction of metallothionein in primary cultures of rat hepatocytes is not mediated through elevation of Zn++uptake.